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1ol0

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Crystal structure of a camelised human VH

Structural highlights

1ol0 is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.8Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The variable domain of dromedary immunoglobulins comprises only the heavy chain and is missing the light-chain variable domain. This single domain is sufficient for antigen recognition and binding-half that required by other mammals. Human antibody-VHs have previously been camelized to be soluble stable fragments that retain antigen binding. Such engineered VHH are of interest in drug development, since they are nonimmunogenic, and in other biotechnology applications. We present the structure of a camelized human antibody fragment (cVH), which is a competitive and reversible inhibitor of the NS3 serine protease of the hepatitis C virus (HCV). In solution, this cVH undergoes a concentration-dependent monomer-dimer equilibrium. The structure confirms the minimum mutational requirements of the VL-binding face. The fragment also suggests a means by which the observed dimerization occurs, highlighting the importance of the composition of the CDR3 in maintaining a truly camelized VH.

Crystal structure of a human VH: requirements for maintaining a monomeric fragment.,Dottorini T, Vaughan CK, Walsh MA, LoSurdo P, Sollazzo M Biochemistry. 2004 Jan 27;43(3):622-8. PMID:14730966^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dottorini T, Vaughan CK, Walsh MA, LoSurdo P, Sollazzo M. Crystal structure of a human VH: requirements for maintaining a monomeric fragment. Biochemistry. 2004 Jan 27;43(3):622-8. PMID:14730966 doi:10.1021/bi035800b

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ol0"

@@ Line 3: / Line 3: @@
 <StructureSection load='1ol0' size='340' side='right'caption='[[1ol0]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ol0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OL0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OL0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ol0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OL0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OL0 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1a8j|1a8j]], [[1aj7|1aj7]], [[1ar2|1ar2]], [[1b6d|1b6d]], [[1bm3|1bm3]], [[1bre|1bre]], [[1bww|1bww]], [[1cmo|1cmo]], [[1ek3|1ek3]], [[1epf|1epf]], [[1fig|1fig]], [[1ie5|1ie5]], [[1igm|1igm]], [[1ikf|1ikf]], [[1ind|1ind]], [[1ine|1ine]], [[1ivl|1ivl]], [[1jgl|1jgl]], [[1jvk|1jvk]], [[1koa|1koa]], [[1ksr|1ksr]], [[1lve|1lve]], [[1mcb|1mcb]], [[1mcc|1mcc]], [[1mcd|1mcd]], [[1mce|1mce]], [[1mcf|1mcf]], [[1mch|1mch]], [[1mci|1mci]], [[1mcj|1mcj]], [[1mck|1mck]], [[1mcl|1mcl]], [[1mcn|1mcn]], [[1mco|1mco]], [[1mcp|1mcp]], [[1mcq|1mcq]], [[1mcr|1mcr]], [[1mcs|1mcs]], [[1mcw|1mcw]], [[1rei|1rei]], [[1vkx|1vkx]], [[1wit|1wit]], [[1wiu|1wiu]], [[2dl2|2dl2]], [[2dli|2dli]], [[2fb4|2fb4]], [[2ig2|2ig2]], [[2imm|2imm]], [[2imn|2imn]], [[2loi|2loi]], [[2mcg|2mcg]], [[2mcp|2mcp]], [[2rcs|2rcs]], [[3mcg|3mcg]], [[5lve|5lve]], [[7fab|7fab]], [[8fab|8fab]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ol0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ol0 OCA], [https://pdbe.org/1ol0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ol0 RCSB], [https://www.ebi.ac.uk/pdbsum/1ol0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ol0 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ol0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ol0 OCA], [http://pdbe.org/1ol0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ol0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ol0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ol0 ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 32: / Line 31: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Dottorini, T]]
+[[Category: Dottorini T]]
-[[Category: Losurdo, P]]
+[[Category: Losurdo P]]
-[[Category: Sollazzo, M]]
+[[Category: Sollazzo M]]
-[[Category: Vaughan, C K]]
+[[Category: Vaughan CK]]
-[[Category: Walsh, M A]]
+[[Category: Walsh MA]]
-[[Category: Camelised variable heavy domain]]
-[[Category: Immunoglobulin]]

1ol0

From Proteopedia

Current revision

Crystal structure of a camelised human VH

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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