2y6x

From Proteopedia

(Difference between revisions)

Current revision

Structure of Psb27 from Thermosynechococcus elongatus

Structural highlights

2y6x is a 1 chain structure with sequence from Thermosynechococcus vestitus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.6Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PSB27_THEVB Plays a role in the repair and/or biogenesis of the calcium-manganese-oxide cluster on the lumenal face of the thylakoid membrane. Its presence in a photosystem II (PSII) preparation prevents binding of other extrinsic subunits PsbO, PsbU and PsbV, and thus assembly of calcium-manganese-oxide cluster. Psb27-containing complexes lack oxygen evolving activity and an oxidizable calcium-manganese-oxide cluster, but have a normal reaction center.^[1]

Publication Abstract from PubMed

The biogenesis and oxygen-evolving activity of cyanobacterial Photosystem II (PSII) is dependent on a number of accessory proteins not found in the crystallised dimeric complex. These include Psb27, a small lipoprotein attached to the lumenal side of PSII, which has been assigned a role in regulating the assembly of the Mn(4)Ca cluster catalysing water oxidation. To gain a better understanding of Psb27, we have determined in this study the crystal structure of the soluble domain of Psb27 from Thermosynechococcus elongatus to a resolution of 1.6 A. The structure is a four-helix bundle, similar to the recently published solution structures of Psb27 from Synechocystis PCC 6803 obtained by nuclear magnetic resonance (NMR) spectroscopy. Importantly, the crystal structure presented here helps us resolve the differences between the NMR-derived structural models. Potential binding sites for Psb27 within PSII are discussed in light of recent biochemical data in the literature.

Crystal structure of the Psb27 assembly factor at 1.6 A: implications for binding to Photosystem II.,Michoux F, Takasaka K, Boehm M, Komenda J, Nixon PJ, Murray JW Photosynth Res. 2011 Dec 23. PMID:22193820^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nowaczyk MM, Hebeler R, Schlodder E, Meyer HE, Warscheid B, Rogner M. Psb27, a cyanobacterial lipoprotein, is involved in the repair cycle of photosystem II. Plant Cell. 2006 Nov;18(11):3121-31. Epub 2006 Nov 17. PMID:17114356 doi:http://dx.doi.org/10.1105/tpc.106.042671
↑ Michoux F, Takasaka K, Boehm M, Komenda J, Nixon PJ, Murray JW. Crystal structure of the Psb27 assembly factor at 1.6 A: implications for binding to Photosystem II. Photosynth Res. 2011 Dec 23. PMID:22193820 doi:10.1007/s11120-011-9712-7

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2y6x"

@@ Line 3: / Line 3: @@
 <StructureSection load='2y6x' size='340' side='right'caption='[[2y6x]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2y6x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus Thermosynechococcus elongatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y6X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Y6X FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2y6x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus Thermosynechococcus vestitus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y6X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y6X FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y6x OCA], [http://pdbe.org/2y6x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2y6x RCSB], [http://www.ebi.ac.uk/pdbsum/2y6x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2y6x ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y6x OCA], [https://pdbe.org/2y6x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y6x RCSB], [https://www.ebi.ac.uk/pdbsum/2y6x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y6x ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PSB27_THEEB PSB27_THEEB]] Plays a role in the repair and/or biogenesis of the calcium-manganese-oxide cluster on the lumenal face of the thylakoid membrane. Its presence in a photosystem II (PSII) preparation prevents binding of other extrinsic subunits PsbO, PsbU and PsbV, and thus assembly of calcium-manganese-oxide cluster. Psb27-containing complexes lack oxygen evolving activity and an oxidizable calcium-manganese-oxide cluster, but have a normal reaction center.<ref>PMID:17114356</ref>
+[https://www.uniprot.org/uniprot/PSB27_THEVB PSB27_THEVB] Plays a role in the repair and/or biogenesis of the calcium-manganese-oxide cluster on the lumenal face of the thylakoid membrane. Its presence in a photosystem II (PSII) preparation prevents binding of other extrinsic subunits PsbO, PsbU and PsbV, and thus assembly of calcium-manganese-oxide cluster. Psb27-containing complexes lack oxygen evolving activity and an oxidizable calcium-manganese-oxide cluster, but have a normal reaction center.<ref>PMID:17114356</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 2y6x" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Photosystem II|Photosystem II]]
 == References ==
 <references/>
@@ Line 26: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Thermosynechococcus elongatus]]
+[[Category: Thermosynechococcus vestitus]]
-[[Category: Boehm, M]]
+[[Category: Boehm M]]
-[[Category: Michoux, F]]
+[[Category: Michoux F]]
-[[Category: Murray, J W]]
+[[Category: Murray JW]]
-[[Category: Nixon, P J]]
+[[Category: Nixon PJ]]
-[[Category: Takasaka, K]]
+[[Category: Takasaka K]]
-[[Category: Photosynthesis]]

2y6x

From Proteopedia

Current revision

Structure of Psb27 from Thermosynechococcus elongatus

Structural highlights

Function

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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