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| | <StructureSection load='4a5p' size='340' side='right'caption='[[4a5p]], [[Resolution|resolution]] 3.15Å' scene=''> | | <StructureSection load='4a5p' size='340' side='right'caption='[[4a5p]], [[Resolution|resolution]] 3.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4a5p]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri_5a Shigella flexneri 5a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A5P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4a5p]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri_5a Shigella flexneri 5a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A5P FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.15Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a5p OCA], [http://pdbe.org/4a5p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a5p RCSB], [http://www.ebi.ac.uk/pdbsum/4a5p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a5p ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a5p OCA], [https://pdbe.org/4a5p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a5p RCSB], [https://www.ebi.ac.uk/pdbsum/4a5p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a5p ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MXIA_SHIFL MXIA_SHIFL]] Necessary for the secretion of IPA invasins. | + | [https://www.uniprot.org/uniprot/MXIA_SHIFL MXIA_SHIFL] Necessary for the secretion of IPA invasins. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Shigella flexneri 5a]] | | [[Category: Shigella flexneri 5a]] |
| - | [[Category: Abrusci, P]] | + | [[Category: Abrusci P]] |
| - | [[Category: Deane, J E]] | + | [[Category: Deane JE]] |
| - | [[Category: Friede, M E]] | + | [[Category: Friede ME]] |
| - | [[Category: Johnson, S]] | + | [[Category: Johnson S]] |
| - | [[Category: Lea, S M]] | + | [[Category: Lea SM]] |
| - | [[Category: Roversi, P]] | + | [[Category: Roversi P]] |
| - | [[Category: Tang, C M]] | + | [[Category: Tang CM]] |
| - | [[Category: Vegara-Irigaray, M]] | + | [[Category: Vegara-Irigaray M]] |
| - | [[Category: Export apparatus]]
| + | |
| - | [[Category: Nonamer]]
| + | |
| - | [[Category: Protein transport]]
| + | |
| - | [[Category: Type three secretion]]
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| Structural highlights
Function
MXIA_SHIFL Necessary for the secretion of IPA invasins.
Publication Abstract from PubMed
Type III secretion systems (T3SSs) are bacterial membrane-embedded nanomachines designed to export specifically targeted proteins from the bacterial cytoplasm. Secretion through T3SS is governed by a subset of inner membrane proteins termed the 'export apparatus'. We show that a key member of the Shigella flexneri export apparatus, MxiA, assembles into a ring essential for secretion in vivo. The ring-forming interfaces are well-conserved in both nonflagellar and flagellar homologs, implying that the ring is an evolutionarily conserved feature in these systems. Electron cryo-tomography revealed a T3SS-associated cytoplasmic torus of size and shape corresponding to those of the MxiA ring aligned to the secretion channel located between the secretion pore and the ATPase complex. This defines the molecular architecture of the dominant component of the export apparatus and allows us to propose a model for the molecular mechanisms controlling secretion.
Architecture of the major component of the type III secretion system export apparatus.,Abrusci P, Vergara-Irigaray M, Johnson S, Beeby MD, Hendrixson DR, Roversi P, Friede ME, Deane JE, Jensen GJ, Tang CM, Lea SM Nat Struct Mol Biol. 2013 Jan;20(1):99-104. doi: 10.1038/nsmb.2452. Epub 2012 Dec, 9. PMID:23222644[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Abrusci P, Vergara-Irigaray M, Johnson S, Beeby MD, Hendrixson DR, Roversi P, Friede ME, Deane JE, Jensen GJ, Tang CM, Lea SM. Architecture of the major component of the type III secretion system export apparatus. Nat Struct Mol Biol. 2013 Jan;20(1):99-104. doi: 10.1038/nsmb.2452. Epub 2012 Dec, 9. PMID:23222644 doi:http://dx.doi.org/10.1038/nsmb.2452
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