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5lkf

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Bovine beta-lactoglobulin complex with myristic acid at high pressure (0.55 GPa)

Structural highlights

5lkf is a 1 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.5Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LACB_BOVIN Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.

Publication Abstract from PubMed

A number of studies were devoted to understanding an immunological effect of pressure-treated beta-lactoglobulin. In our previous work we have proved that high pressure significantly modifies beta-lactoglobulin conformation and consequently its physicochemical properties. Here, structure of beta-lactoglobulin complex with myristic acid determined at the highest accepted by the crystal pressure value of 550MPa is reported. Our results structurally prove that pressure noticeably modifies positions of the major beta-lactoglobulin epitopes. Considering the biological impact of observed changes in epitope regions, high pressure beta-lactoglobulin structure presents a step forward in understanding the pressure modification of food protein allergenicity. The conformational changes of pressurized beta-lactoglobulin did not support the hypothesis that proteolytic digestion facilitated by pressure is caused by an exposure of the digestive sites. Our findings demonstrate that high pressure protein crystallography can potentially identify the most pressure-sensitive fragments in allergens, and can therefore support development of hypoallergenic food products.

Towards understanding the effect of high pressure on food protein allergenicity: beta-lactoglobulin structural studies.,Kurpiewska K, Biela A, Loch JI, Lipowska J, Siuda M, Lewinski K Food Chem. 2019 Jan 1;270:315-321. doi: 10.1016/j.foodchem.2018.07.104. Epub 2018, Jul 17. PMID:30174052^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kurpiewska K, Biela A, Loch JI, Lipowska J, Siuda M, Lewinski K. Towards understanding the effect of high pressure on food protein allergenicity: beta-lactoglobulin structural studies. Food Chem. 2019 Jan 1;270:315-321. doi: 10.1016/j.foodchem.2018.07.104. Epub 2018, Jul 17. PMID:30174052 doi:http://dx.doi.org/10.1016/j.foodchem.2018.07.104

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5lkf"

Categories: Bos taurus | Large Structures | Garbacz K | Kurpiewska K | Lewinski K

@@ Line 3: / Line 3: @@
 <StructureSection load='5lkf' size='340' side='right'caption='[[5lkf]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5lkf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LKF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LKF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5lkf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LKF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LKF FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5lke|5lke]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lkf OCA], [http://pdbe.org/5lkf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lkf RCSB], [http://www.ebi.ac.uk/pdbsum/5lkf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lkf ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lkf OCA], [https://pdbe.org/5lkf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lkf RCSB], [https://www.ebi.ac.uk/pdbsum/5lkf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lkf ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN]] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
+[https://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 28: / Line 28: @@
 [[Category: Bos taurus]]
 [[Category: Large Structures]]
-[[Category: Garbacz, K]]
+[[Category: Garbacz K]]
-[[Category: Kurpiewska, K]]
+[[Category: Kurpiewska K]]
-[[Category: Lewinski, K]]
+[[Category: Lewinski K]]
-[[Category: Beta-lactoglobulin]]
-[[Category: Lipocalin]]
-[[Category: Transport protein]]

5lkf

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Current revision

Bovine beta-lactoglobulin complex with myristic acid at high pressure (0.55 GPa)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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