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| | <StructureSection load='6ian' size='340' side='right'caption='[[6ian]], [[Resolution|resolution]] 3.20Å' scene=''> | | <StructureSection load='6ian' size='340' side='right'caption='[[6ian]], [[Resolution|resolution]] 3.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6ian]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Tryb2 Tryb2] and [http://en.wikipedia.org/wiki/Trypanosoma_brucei_equiperdum Trypanosoma brucei equiperdum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IAN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ian]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei_TREU927 Trypanosoma brucei brucei TREU927] and [https://en.wikipedia.org/wiki/Trypanosoma_brucei_equiperdum Trypanosoma brucei equiperdum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IAN FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tb927.7.3370 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=185431 TRYB2]), Tb10.70.5020 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=185431 TRYB2]), RABL5, DPX39_110145000 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=630700 Trypanosoma brucei equiperdum])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ian FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ian OCA], [https://pdbe.org/6ian PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ian RCSB], [https://www.ebi.ac.uk/pdbsum/6ian PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ian ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ian FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ian OCA], [http://pdbe.org/6ian PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ian RCSB], [http://www.ebi.ac.uk/pdbsum/6ian PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ian ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q57WF2_TRYB2 Q57WF2_TRYB2] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Tryb2]] | + | [[Category: Trypanosoma brucei brucei TREU927]] |
| | [[Category: Trypanosoma brucei equiperdum]] | | [[Category: Trypanosoma brucei equiperdum]] |
| - | [[Category: Basquin, J]] | + | [[Category: Basquin J]] |
| - | [[Category: Lorentzen, E]] | + | [[Category: Lorentzen E]] |
| - | [[Category: Wachter, S]] | + | [[Category: Wachter S]] |
| - | [[Category: Cilium formation]]
| + | |
| - | [[Category: Cytosolic protein]]
| + | |
| - | [[Category: Gtpase]]
| + | |
| - | [[Category: Ift74]]
| + | |
| - | [[Category: Ift81]]
| + | |
| - | [[Category: Intraflagellar transport protein 22]]
| + | |
| - | [[Category: Rab-like]]
| + | |
| - | [[Category: Rabl5]]
| + | |
| Structural highlights
Function
Q57WF2_TRYB2
Publication Abstract from PubMed
Intraflagellar transport (IFT) relies on motor proteins and the IFT complex to construct cilia and flagella. The IFT complex subunit IFT22/RabL5 has sequence similarity with small GTPases although the nucleotide specificity is unclear because of non-conserved G4/G5 motifs. We show that IFT22 specifically associates with G-nucleotides and present crystal structures of IFT22 in complex with GDP, GTP, and with IFT74/81. Our structural analysis unravels an unusual GTP/GDP-binding mode of IFT22 bypassing the classical G4 motif. The GTPase switch regions of IFT22 become ordered upon complex formation with IFT74/81 and mediate most of the IFT22-74/81 interactions. Structure-based mutagenesis reveals that association of IFT22 with the IFT complex is essential for flagellum construction in Trypanosoma brucei although IFT22 GTP-loading is not strictly required.
Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly.,Wachter S, Jung J, Shafiq S, Basquin J, Fort C, Bastin P, Lorentzen E EMBO J. 2019 Apr 2. pii: embj.2018101251. doi: 10.15252/embj.2018101251. PMID:30940671[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wachter S, Jung J, Shafiq S, Basquin J, Fort C, Bastin P, Lorentzen E. Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly. EMBO J. 2019 Apr 2. pii: embj.2018101251. doi: 10.15252/embj.2018101251. PMID:30940671 doi:http://dx.doi.org/10.15252/embj.2018101251
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