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| | ==THE SOLUTION STRUCTURE OF OMEGA-AGA-IVB, A P-TYPE CALCIUM CHANNEL ANTAGONIST FROM THE VENOM OF AGELENOPSIS APERTA== | | ==THE SOLUTION STRUCTURE OF OMEGA-AGA-IVB, A P-TYPE CALCIUM CHANNEL ANTAGONIST FROM THE VENOM OF AGELENOPSIS APERTA== |
| - | <StructureSection load='1agg' size='340' side='right'caption='[[1agg]], [[NMR_Ensembles_of_Models | 24 NMR models]]' scene=''> | + | <StructureSection load='1agg' size='340' side='right'caption='[[1agg]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1agg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Agelenopsis_aperta Agelenopsis aperta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AGG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AGG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1agg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Agelenopsis_aperta Agelenopsis aperta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AGG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AGG FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1agg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1agg OCA], [http://pdbe.org/1agg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1agg RCSB], [http://www.ebi.ac.uk/pdbsum/1agg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1agg ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1agg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1agg OCA], [https://pdbe.org/1agg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1agg RCSB], [https://www.ebi.ac.uk/pdbsum/1agg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1agg ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TOG4B_AGEAP TOG4B_AGEAP]] Antagonist of voltage-gated Cav2.1/CACNA1A (P-type) calcium channels. Paralyzes insect by blocking neuromuscular transmission.<ref>PMID:8232218</ref> | + | [https://www.uniprot.org/uniprot/TX23B_AGEAP TX23B_AGEAP] Antagonist of voltage-gated Cav2.1/CACNA1A (P-type) calcium channels. Paralyzes insect by blocking neuromuscular transmission.<ref>PMID:8232218</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Agelenopsis aperta]] | | [[Category: Agelenopsis aperta]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Adams, M E]] | + | [[Category: Adams ME]] |
| - | [[Category: Reily, M D]] | + | [[Category: Reily MD]] |
| - | [[Category: Thanabal, V]] | + | [[Category: Thanabal V]] |
| - | [[Category: Neurotoxin]]
| + | |
| - | [[Category: P-type calcium channel antagonist]]
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| Structural highlights
Function
TX23B_AGEAP Antagonist of voltage-gated Cav2.1/CACNA1A (P-type) calcium channels. Paralyzes insect by blocking neuromuscular transmission.[1]
Publication Abstract from PubMed
The 48 amino acid peptides omega-Aga-IVA and omega-Aga-IVB are the first agents known to specifically block P-type calcium channels in mammalian brain, thus complementing the existing suite of pharmacological tools used for characterizing calcium channels. These peptides provide a new set of probes for studies aimed at elucidating the structural basis underlying the subtype specificity of calcium channel antagonists. We used 288 NMR-derived constraints in a protocol combining distance geometry and molecular dynamics employing the program DGII, followed by energy minimization with Discover to derive the three-dimensional structure of omega-Aga-IVB. The toxin consists of a well-defined core region, comprising seven solvent-shielded residues and a well-defined triple-stranded beta-sheet. Four loop regions have average backbone rms deviations between 0.38 and 1.31 A, two of which are well-defined type-II beta-turns. Other structural features include disordered C- and N-termini and several conserved basic amino acids that are clustered on one face of the molecule. The reported structure suggests a possible surface for interaction with the channel. This surface contains amino acids that are identical to those of another known P-type calcium channel antagonist, omega-Aga-IVA, and is rich in basic residues that may have a role in binding to the anionic sites in the extracellular regions of the calcium channel.
The solution structure of omega-Aga-IVB, a P-type calcium channel antagonist from venom of the funnel web spider, Agelenopsis aperta.,Reily MD, Thanabal V, Adams ME J Biomol NMR. 1995 Feb;5(2):122-32. PMID:7703698[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Adams ME, Mintz IM, Reily MD, Thanabal V, Bean BP. Structure and properties of omega-agatoxin IVB, a new antagonist of P-type calcium channels. Mol Pharmacol. 1993 Oct;44(4):681-8. PMID:8232218
- ↑ Reily MD, Thanabal V, Adams ME. The solution structure of omega-Aga-IVB, a P-type calcium channel antagonist from venom of the funnel web spider, Agelenopsis aperta. J Biomol NMR. 1995 Feb;5(2):122-32. PMID:7703698
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