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| | <StructureSection load='4u6b' size='340' side='right'caption='[[4u6b]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='4u6b' size='340' side='right'caption='[[4u6b]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4u6b]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"cytophaga_drobachiensis"_barbeyron_et_al._1998 "cytophaga drobachiensis" barbeyron et al. 1998]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U6B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U6B FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4u6b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Zobellia_galactanivorans Zobellia galactanivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U6B FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ak5|4ak5]], [[3p2n|3p2n]], [[3r4y|3r4y]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">zobellia_3597 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63186 "Cytophaga drobachiensis" Barbeyron et al. 1998])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u6b OCA], [https://pdbe.org/4u6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u6b RCSB], [https://www.ebi.ac.uk/pdbsum/4u6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u6b ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u6b OCA], [http://pdbe.org/4u6b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4u6b RCSB], [http://www.ebi.ac.uk/pdbsum/4u6b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4u6b ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/F0V1E1_ZOBGA F0V1E1_ZOBGA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cytophaga drobachiensis barbeyron et al. 1998]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ficko-Blean, E]] | + | [[Category: Zobellia galactanivorans]] |
| - | [[Category: Gh117]] | + | [[Category: Ficko-Blean E]] |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
4u6b is a 4 chain structure with sequence from Zobellia galactanivorans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 2.3Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
F0V1E1_ZOBGA
Publication Abstract from PubMed
The family 117 glycoside hydrolase (GH117) enzymes have exo-alpha-1,3-(3,6-anhydro)-L-galactosidase activity, removing terminal nonreducing alpha-1,3-linked 3,6-anhydro-L-galactose residues from their red algal neoagarose substrate. These enzymes have previously been phylogenetically divided into clades, and only the clade A enzymes have been experimentally studied to date. The investigation of two GH117 enzymes, Zg3615 and Zg3597, produced by the marine bacterium Zobellia galactanivorans reveals structural, biochemical and further phylogenetic diversity between clades. A product complex with the unusual beta-3,6-anhydro-L-galactose residue sheds light on the inverting catalytic mechanism of the GH117 enzymes as well as the structure of this unique sugar produced by hydrolysis of the agarophyte red algal cell wall.
Biochemical and structural investigation of two paralogous glycoside hydrolases from Zobellia galactanivorans: novel insights into the evolution, dimerization plasticity and catalytic mechanism of the GH117 family.,Ficko-Blean E, Duffieux D, Rebuffet E, Larocque R, Groisillier A, Michel G, Czjzek M Acta Crystallogr D Biol Crystallogr. 2015 Feb;71(Pt 2):209-23. doi:, 10.1107/S1399004714025024. Epub 2015 Jan 23. PMID:25664732[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ficko-Blean E, Duffieux D, Rebuffet E, Larocque R, Groisillier A, Michel G, Czjzek M. Biochemical and structural investigation of two paralogous glycoside hydrolases from Zobellia galactanivorans: novel insights into the evolution, dimerization plasticity and catalytic mechanism of the GH117 family. Acta Crystallogr D Biol Crystallogr. 2015 Feb;71(Pt 2):209-23. doi:, 10.1107/S1399004714025024. Epub 2015 Jan 23. PMID:25664732 doi:http://dx.doi.org/10.1107/S1399004714025024
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