6ovp
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6ovp is ON HOLD Authors: Gianotti, A.R., Klinke, S., Ermacora, M.R. Description: Sterol Carrier Protein 2 from Yarrowia Lipolytica (apo form) [[Cat...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Sterol Carrier Protein 2 from Yarrowia Lipolytica (apo form)== | |
| + | <StructureSection load='6ovp' size='340' side='right'caption='[[6ovp]], [[Resolution|resolution]] 1.99Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6ovp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Yarrowia_lipolytica Yarrowia lipolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OVP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OVP FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.991Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ovp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ovp OCA], [https://pdbe.org/6ovp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ovp RCSB], [https://www.ebi.ac.uk/pdbsum/6ovp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ovp ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SCP2_YARLI SCP2_YARLI] May play a role in the transport of fatty acids. Binds fatty acids and fatty acyl-CoAs including palmitic acid, oleic acid, cis-parinaric acid and palmitoyl-CoA (in vitro).<ref>PMID:16890184</ref> <ref>PMID:8828794</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Isolated or as a part of multidomain proteins, Sterol Carrier Protein 2 (SCP2) exhibits high affinity and broad specificity for different lipidic and hydrophobic compounds. A wealth of structural information on SCP2 domains in all forms of life is currently available; however, many aspects of its ligand binding activity are poorly understood. ylSCP2 is a well-characterized single domain SCP2 from the yeast Yarrowia lipolytica. Herein, we report the X-ray structure of unliganded ylSCP2 refined to 2.0 A resolution. Comparison with the previously solved liganded ylSCP2 structure unveiled a novel mechanism for binding site occlusion. The liganded ylSCP2 binding site is a large cavity with a volume of more than 800 A(3). In unliganded ylSCP2 the binding site is reduced to about 140 A(3). The obliteration is caused by a swing movement of the C-terminal alpha helix 5 and a subtle compaction of helices 2-4. Previous pairwise comparisons were between homologous SCP2 domains with a uncertain binding status. The reported unliganded ylSCP2 structure allows for the first time a fully controlled comparative analysis of the conformational effects of ligand occupation dispelling several doubts regarding the architecture of SCP2 binding site. | ||
| - | + | The structure of unliganded sterol carrier protein 2 from Yarrowia lipolytica unveils a mechanism for binding site occlusion.,Gianotti AR, Klinke S, Ermacora MR J Struct Biol. 2020 Dec 2;213(1):107675. doi: 10.1016/j.jsb.2020.107675. PMID:33278583<ref>PMID:33278583</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6ovp" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: Gianotti | + | ==See Also== |
| + | *[[Fatty acid-binding protein 3D structures|Fatty acid-binding protein 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Yarrowia lipolytica]] | ||
| + | [[Category: Ermacora MR]] | ||
| + | [[Category: Gianotti AR]] | ||
| + | [[Category: Klinke S]] | ||
Current revision
Sterol Carrier Protein 2 from Yarrowia Lipolytica (apo form)
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