6rnf
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6rnf is ON HOLD until sometime in the future Authors: Mehrabi, P., Schulz, E.C., Miller, R.J.D. Description: Liquid Application Method for time-res...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Xylose Isomerase 30 ms timepoint== | |
| + | <StructureSection load='6rnf' size='340' side='right'caption='[[6rnf]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6rnf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_rubiginosus Streptomyces rubiginosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RNF FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rnf OCA], [https://pdbe.org/6rnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rnf RCSB], [https://www.ebi.ac.uk/pdbsum/6rnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rnf ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/XYLA_STRRU XYLA_STRRU] Involved in D-xylose catabolism. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase. | ||
| - | + | Liquid application method for time-resolved analyses by serial synchrotron crystallography.,Mehrabi P, Schulz EC, Agthe M, Horrell S, Bourenkov G, von Stetten D, Leimkohl JP, Schikora H, Schneider TR, Pearson AR, Tellkamp F, Miller RJD Nat Methods. 2019 Oct;16(10):979-982. doi: 10.1038/s41592-019-0553-1. Epub 2019, Sep 16. PMID:31527838<ref>PMID:31527838</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Mehrabi | + | <div class="pdbe-citations 6rnf" style="background-color:#fffaf0;"></div> |
| - | [[Category: Miller | + | |
| - | [[Category: Schulz | + | ==See Also== |
| + | *[[D-xylose isomerase 3D structures|D-xylose isomerase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Streptomyces rubiginosus]] | ||
| + | [[Category: Mehrabi P]] | ||
| + | [[Category: Miller RJD]] | ||
| + | [[Category: Schulz EC]] | ||
Current revision
Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Xylose Isomerase 30 ms timepoint
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