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| | <StructureSection load='2w1j' size='340' side='right'caption='[[2w1j]], [[Resolution|resolution]] 1.24Å' scene=''> | | <StructureSection load='2w1j' size='340' side='right'caption='[[2w1j]], [[Resolution|resolution]] 1.24Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2w1j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpn Strpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W1J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W1J FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2w1j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W1J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W1J FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.24Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2w1k|2w1k]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidyltransferase Peptidyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.12 2.3.2.12] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w1j OCA], [https://pdbe.org/2w1j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w1j RCSB], [https://www.ebi.ac.uk/pdbsum/2w1j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w1j ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w1j OCA], [http://pdbe.org/2w1j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w1j RCSB], [http://www.ebi.ac.uk/pdbsum/2w1j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w1j ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0H2UNQ9_STRPN A0A0H2UNQ9_STRPN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Peptidyltransferase]] | + | [[Category: Streptococcus pneumoniae TIGR4]] |
| - | [[Category: Strpn]]
| + | [[Category: Contreras-Martel C]] |
| - | [[Category: Contreras-Martel, C]] | + | [[Category: Dessen A]] |
| - | [[Category: Dessen, A]] | + | [[Category: Di Guilmi AM]] |
| - | [[Category: Fenel, D]] | + | [[Category: El Mortaji L]] |
| - | [[Category: Guilmi, A M.Di]] | + | [[Category: Fenel D]] |
| - | [[Category: Izore, T]] | + | [[Category: Izore T]] |
| - | [[Category: Manzano, C]] | + | [[Category: Manzano C]] |
| - | [[Category: Mortaji, L El]] | + | [[Category: Schoehn G]] |
| - | [[Category: Schoehn, G]] | + | [[Category: Vernet T]] |
| - | [[Category: Vernet, T]] | + | |
| - | [[Category: Pathogenicity]]
| + | |
| - | [[Category: Transferase]]
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| Structural highlights
Function
A0A0H2UNQ9_STRPN
Publication Abstract from PubMed
Streptococcus pneumoniae is a piliated pathogen whose ability to circumvent vaccination and antibiotic treatment strategies is a cause of mortality worldwide. Pili play important roles in pneumococcal infection, but little is known about their biogenesis mechanism or the relationship between components of the pilus-forming machinery, which includes the fiber pilin (RrgB), two minor pilins (RrgA, RrgC), and three sortases (SrtC-1, SrtC-2, SrtC-3). Here we show that SrtC-1 is the main pilus-polymerizing transpeptidase, and electron microscopy analyses of RrgB fibers reconstituted in vitro reveal that they structurally mimic the pneumococcal pilus backbone. Crystal structures of both SrtC-1 and SrtC-3 reveal active sites whose access is controlled by flexible lids, unlike in non-pilus sortases, and suggest that substrate specificity is dictated by surface recognition coupled to lid opening. The distinct structural features of pilus-forming sortases suggest a common pilus biogenesis mechanism that could be exploited for the development of broad-spectrum antibacterials.
Sortase-Mediated Pilus Fiber Biogenesis in Streptococcus pneumoniae.,Manzano C, Contreras-Martel C, El Mortaji L, Izore T, Fenel D, Vernet T, Schoehn G, Di Guilmi AM, Dessen A Structure. 2008 Dec 12;16(12):1838-48. PMID:19081060[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Manzano C, Contreras-Martel C, El Mortaji L, Izore T, Fenel D, Vernet T, Schoehn G, Di Guilmi AM, Dessen A. Sortase-Mediated Pilus Fiber Biogenesis in Streptococcus pneumoniae. Structure. 2008 Dec 12;16(12):1838-48. PMID:19081060 doi:S0969-2126(08)00385-7
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