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4up8

From Proteopedia

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Crystal structure of Entamoeba histolytica lysyl-tRNA synthetase apo form

Structural highlights

4up8 is a 1 chain structure with sequence from Entamoeba histolytica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.896Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

C4M7X2_ENTH1

Publication Abstract from PubMed

The class II lysyl-tRNA synthetases (KRS) are conserved aminoacyl-tRNA synthetases that attach lysine to the cognate tRNA in a two-step mechanism. The enzyme from the parasitic protozoan Entamoeba histolytica was crystallized in the presence of small ligands to generate snapshots of the lysine-adenylate formation. The residues involved in lysine activation are highly conserved and the active site closes around the lysyl-adenylate, as observed in bacterial KRS. The Entamoeba EMAPII-like polypeptide is not resolved in the crystals, but another Entamoeba-specific insertion could be modeled as a small helix bundle that may contribute to tRNA binding through interaction with the tRNA hinge.

Crystal structures of Entamoeba histolytica lysyl-tRNA synthetase reveal conformational changes upon lysine binding and a specific helix bundle domain.,Bonnefond L, Castro de Moura M, Ribas de Pouplana L, Nureki O FEBS Lett. 2014 Oct 24;588(23):4478-4486. doi: 10.1016/j.febslet.2014.10.019. PMID:25448989^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bonnefond L, Castro de Moura M, Ribas de Pouplana L, Nureki O. Crystal structures of Entamoeba histolytica lysyl-tRNA synthetase reveal conformational changes upon lysine binding and a specific helix bundle domain. FEBS Lett. 2014 Oct 24;588(23):4478-4486. doi: 10.1016/j.febslet.2014.10.019. PMID:25448989 doi:http://dx.doi.org/10.1016/j.febslet.2014.10.019

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4up8"

Categories: Entamoeba histolytica | Large Structures | Bonnefond L | Nureki O

@@ Line 3: / Line 3: @@
 <StructureSection load='4up8' size='340' side='right'caption='[[4up8]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4up8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enthi Enthi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UP8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UP8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4up8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UP8 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4up7|4up7]], [[4up9|4up9]], [[4upa|4upa]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.896&#8491;</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysine--tRNA_ligase Lysine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.6 6.1.1.6] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4up8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4up8 OCA], [https://pdbe.org/4up8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4up8 RCSB], [https://www.ebi.ac.uk/pdbsum/4up8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4up8 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4up8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4up8 OCA], [http://pdbe.org/4up8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4up8 RCSB], [http://www.ebi.ac.uk/pdbsum/4up8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4up8 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/C4M7X2_ENTH1 C4M7X2_ENTH1]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: Enthi]]
+[[Category: Entamoeba histolytica]]
 [[Category: Large Structures]]
-[[Category: Lysine--tRNA ligase]]
+[[Category: Bonnefond L]]
-[[Category: Bonnefond, L]]
+[[Category: Nureki O]]
-[[Category: Nureki, O]]
-[[Category: Aminoacylation]]
-[[Category: Ligase]]

4up8

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Current revision

Crystal structure of Entamoeba histolytica lysyl-tRNA synthetase apo form

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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