2j0x

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[[Image:2j0x.gif|left|200px]]<br />
 
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<applet load="2j0x" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2j0x, resolution 2.80&Aring;" />
 
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'''CRYSTAL STRUCTURE OF E. COLI ASPARTOKINASE III IN COMPLEX WITH LYSINE AND ASPARTATE (T-STATE)'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF E. COLI ASPARTOKINASE III IN COMPLEX WITH LYSINE AND ASPARTATE (T-STATE)==
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Aspartokinase III (AKIII) from Escherichia coli catalyzes an initial, commitment step of the aspartate pathway, giving biosynthesis of certain, amino acids including lysine. We report crystal structures of AKIII in the, inactive T-state with bound feedback allosteric inhibitor lysine and in, the R-state with aspartate and ADP. The structures reveal an unusual, configuration for the regulatory ACT domains, in which ACT2 is inserted, into ACT1 rather than the expected tandem repeat. Comparison of R- and, T-state AKIII indicates that binding of lysine to the regulatory ACT1, domain in R-state AKIII instigates a series of changes that release a, "latch", the beta15-alphaK loop, from the catalytic domain, which in turn, undergoes large rotational rearrangements, promoting tetramer formation, and completion of the transition to the T-state. Lysine-induced allosteric, transition in AKIII involves both destabilizing the R-state and, stabilizing the T-state tetramer. Rearrangement of the catalytic domain, blocks the ATP-binding site, which is therefore the structural basis for, allosteric inhibition of AKIII by lysine.
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<StructureSection load='2j0x' size='340' side='right'caption='[[2j0x]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2j0x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J0X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J0X FirstGlance]. <br>
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2J0X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PO4, ASP and LYS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Aspartate_kinase Aspartate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.4 2.7.2.4] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J0X OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j0x OCA], [https://pdbe.org/2j0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j0x RCSB], [https://www.ebi.ac.uk/pdbsum/2j0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j0x ProSAT]</span></td></tr>
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Structures of R- and T-state Escherichia coli aspartokinase III. Mechanisms of the allosteric transition and inhibition by lysine., Kotaka M, Ren J, Lockyer M, Hawkins AR, Stammers DK, J Biol Chem. 2006 Oct 20;281(42):31544-52. Epub 2006 Aug 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16905770 16905770]
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</table>
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[[Category: Aspartate kinase]]
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== Function ==
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[https://www.uniprot.org/uniprot/AK3_ECOLI AK3_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j0/2j0x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j0x ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hawkins, A.R.]]
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[[Category: Hawkins AR]]
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[[Category: Kotaka, M.]]
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[[Category: Kotaka M]]
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[[Category: Lockyer, M.]]
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[[Category: Lockyer M]]
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[[Category: Ren, J.]]
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[[Category: Ren J]]
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[[Category: Stammers, D.K.]]
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[[Category: Stammers DK]]
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[[Category: ASP]]
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[[Category: LYS]]
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[[Category: PO4]]
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[[Category: act domain]]
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[[Category: allosteric regulation]]
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[[Category: amino acid biosynthesis]]
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[[Category: amino-acid biosynthesis]]
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[[Category: aspartate pathway]]
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[[Category: aspartokinase]]
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[[Category: feedback inhibition]]
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[[Category: kinase]]
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[[Category: lysine]]
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[[Category: lysine biosynthesis]]
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[[Category: transferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 18:22:21 2007''
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Current revision

CRYSTAL STRUCTURE OF E. COLI ASPARTOKINASE III IN COMPLEX WITH LYSINE AND ASPARTATE (T-STATE)

PDB ID 2j0x

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