4wx0

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<StructureSection load='4wx0' size='340' side='right'caption='[[4wx0]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
<StructureSection load='4wx0' size='340' side='right'caption='[[4wx0]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4wx0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psef5 Psef5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WX0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WX0 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4wx0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_protegens_Pf-5 Pseudomonas protegens Pf-5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WX0 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HXD:(3R)-3-HYDROXYDODECANOIC+ACID'>HXD</scene>, <scene name='pdbligand=PER:PEROXIDE+ION'>PER</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4wwj|4wwj]], [[4wwz|4wwz]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HXD:(3R)-3-HYDROXYDODECANOIC+ACID'>HXD</scene>, <scene name='pdbligand=PER:PEROXIDE+ION'>PER</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PFL_4321 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=220664 PSEF5])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wx0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wx0 OCA], [https://pdbe.org/4wx0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wx0 RCSB], [https://www.ebi.ac.uk/pdbsum/4wx0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wx0 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wx0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wx0 OCA], [http://pdbe.org/4wx0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wx0 RCSB], [http://www.ebi.ac.uk/pdbsum/4wx0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wx0 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q4K8M0_PSEF5 Q4K8M0_PSEF5]
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Aliphatic medium-chain 1-alkenes (MCAEs, approximately 10 carbons) are "drop-in" compatible next-generation fuels and precursors to commodity chemicals. Mass production of MCAEs from renewable resources holds promise for mitigating dependence on fossil hydrocarbons. An MCAE, such as 1-undecene, is naturally produced by Pseudomonas as a semivolatile metabolite through an unknown biosynthetic pathway. We describe here the discovery of a single gene conserved in Pseudomonas responsible for 1-undecene biosynthesis. The encoded enzyme is able to convert medium-chain fatty acids (C10-C14) into their corresponding terminal olefins using an oxygen-activating, nonheme iron-dependent mechanism. Both biochemical and X-ray crystal structural analyses suggest an unusual mechanism of beta-hydrogen abstraction during fatty acid substrate activation. Our discovery unveils previously unidentified chemistry in the nonheme Fe(II) enzyme family, provides an opportunity to explore the biology of 1-undecene in Pseudomonas, and paves the way for tailored bioconversion of renewable raw materials to MCAE-based biofuels and chemical commodities.
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Microbial biosynthesis of medium-chain 1-alkenes by a nonheme iron oxidase.,Rui Z, Li X, Zhu X, Liu J, Domigan B, Barr I, Cate JH, Zhang W Proc Natl Acad Sci U S A. 2014 Dec 8. pii: 201419701. PMID:25489112<ref>PMID:25489112</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4wx0" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Psef5]]
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[[Category: Pseudomonas protegens Pf-5]]
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[[Category: Cate, J D.H]]
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[[Category: Cate JDH]]
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[[Category: Li, X]]
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[[Category: Li X]]
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[[Category: 1-undecene biosynthesis]]
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[[Category: Aliphatic medium-chain 1-alkene biosynthesis]]
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[[Category: Metalloenzyme]]
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[[Category: Non-heme iron dependent desaturase/decarboxylase]]
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[[Category: Oxidoreductase]]
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Current revision

UndA complexed with beta-hydroxydodecanoic acid

PDB ID 4wx0

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