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| | <StructureSection load='4wd7' size='340' side='right'caption='[[4wd7]], [[Resolution|resolution]] 2.90Å' scene=''> | | <StructureSection load='4wd7' size='340' side='right'caption='[[4wd7]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4wd7]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae_uhkpc96 Klebsiella pneumoniae uhkpc96]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WD7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WD7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4wd7]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae_UHKPC96 Klebsiella pneumoniae UHKPC96]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WD7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WD7 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">H215_4284 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1284795 Klebsiella pneumoniae UHKPC96])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wd7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wd7 OCA], [http://pdbe.org/4wd7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wd7 RCSB], [http://www.ebi.ac.uk/pdbsum/4wd7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wd7 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wd7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wd7 OCA], [https://pdbe.org/4wd7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wd7 RCSB], [https://www.ebi.ac.uk/pdbsum/4wd7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wd7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4wd7" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4wd7" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Bestrophin 3D structures|Bestrophin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Klebsiella pneumoniae uhkpc96]] | + | [[Category: Klebsiella pneumoniae UHKPC96]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hendrickson, W A]] | + | [[Category: Hendrickson WA]] |
| - | [[Category: Liu, Q]] | + | [[Category: Liu Q]] |
| - | [[Category: NYCOMPS, New York Consortium on Membrane Protein Structure]]
| + | [[Category: Yang T]] |
| - | [[Category: Yang, T]] | + | |
| - | [[Category: Calcium-activated chloride channel]]
| + | |
| - | [[Category: Macular degeneration]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: New york consortium on membrane protein structure]]
| + | |
| - | [[Category: Nycomp]]
| + | |
| - | [[Category: Pentamer]]
| + | |
| - | [[Category: Psi-biology]]
| + | |
| - | [[Category: Sodium channel]]
| + | |
| - | [[Category: Structural genomic]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Human bestrophin-1 (hBest1) is a calcium-activated chloride channel from the retinal pigment epithelium, where mutations are associated with vitelliform macular degeneration, or Best disease. We describe the structure of a bacterial homolog (KpBest) of hBest1 and functional characterizations of both channels. KpBest is a pentamer that forms a five-helix transmembrane pore, closed by three rings of conserved hydrophobic residues, and has a cytoplasmic cavern with a restricted exit. From electrophysiological analysis of structure-inspired mutations in KpBest and hBest1, we find a sensitive control of ion selectivity in the bestrophins, including reversal of anion/cation selectivity, and dramatic activation by mutations at the cytoplasmic exit. A homology model of hBest1 shows the locations of disease-causing mutations and suggests possible roles in regulation.
Structure and selectivity in bestrophin ion channels.,Yang T, Liu Q, Kloss B, Bruni R, Kalathur RC, Guo Y, Kloppmann E, Rost B, Colecraft HM, Hendrickson WA Science. 2014 Oct 17;346(6207):355-9. doi: 10.1126/science.1259723. Epub 2014 Sep, 25. PMID:25324390[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Yang T, Liu Q, Kloss B, Bruni R, Kalathur RC, Guo Y, Kloppmann E, Rost B, Colecraft HM, Hendrickson WA. Structure and selectivity in bestrophin ion channels. Science. 2014 Oct 17;346(6207):355-9. doi: 10.1126/science.1259723. Epub 2014 Sep, 25. PMID:25324390 doi:http://dx.doi.org/10.1126/science.1259723
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