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| | <StructureSection load='4wwd' size='340' side='right'caption='[[4wwd]], [[Resolution|resolution]] 1.30Å' scene=''> | | <StructureSection load='4wwd' size='340' side='right'caption='[[4wwd]], [[Resolution|resolution]] 1.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4wwd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cupmc Cupmc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WWD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4wwd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_metallidurans_CH34 Cupriavidus metallidurans CH34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WWD FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=CO2:CARBON+DIOXIDE'>CO2</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cnrR, cnrX, Rmet_6206, RMe0087 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=266264 CUPMC])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=CO2:CARBON+DIOXIDE'>CO2</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwd OCA], [http://pdbe.org/4wwd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wwd RCSB], [http://www.ebi.ac.uk/pdbsum/4wwd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wwd ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwd OCA], [https://pdbe.org/4wwd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wwd RCSB], [https://www.ebi.ac.uk/pdbsum/4wwd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wwd ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CNRR_RALME CNRR_RALME]] CnrH alone is able to activate cnr expression, while both CnrY and CrnR (CnrX) are needed for nickel induction of CnrH (PubMed:10671463). Has been suggested (PubMed:10671464) to bind nickel. | + | [https://www.uniprot.org/uniprot/CNRR_CUPMC CNRR_CUPMC] CnrH alone is able to activate cnr expression, while both CnrY and CrnR (CnrX) are needed for nickel induction of CnrH (PubMed:10671463). Has been suggested (PubMed:10671464) to bind nickel.<ref>PMID:10671463</ref> <ref>PMID:10671464</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cupmc]] | + | [[Category: Cupriavidus metallidurans CH34]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Coves, J]] | + | [[Category: Coves J]] |
| - | [[Category: Grosse, C]] | + | [[Category: Grosse C]] |
| - | [[Category: Kuennemann, S]] | + | [[Category: Kuennemann S]] |
| - | [[Category: Maillard, A P]] | + | [[Category: Maillard AP]] |
| - | [[Category: Nies, D H]] | + | [[Category: Nies DH]] |
| - | [[Category: Petit-Haertlein, I]] | + | [[Category: Petit-Haertlein I]] |
| - | [[Category: Rosny, E de]]
| + | [[Category: Schleuder G]] |
| - | [[Category: Schleuder, G]] | + | [[Category: Volbeda A]] |
| - | [[Category: Volbeda, A]] | + | [[Category: De Rosny E]] |
| - | [[Category: Metal binding protein]] | + | |
| - | [[Category: Nickel sensor]]
| + | |
| Structural highlights
4wwd is a 1 chain structure with sequence from Cupriavidus metallidurans CH34. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 1.3Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
CNRR_CUPMC CnrH alone is able to activate cnr expression, while both CnrY and CrnR (CnrX) are needed for nickel induction of CnrH (PubMed:10671463). Has been suggested (PubMed:10671464) to bind nickel.[1] [2]
Publication Abstract from PubMed
Resistance to high concentration of nickel ions is mediated in Cupriavidus metallidurans by the CnrCBA transenvelope efflux complex. Expression of the cnrCBA genes is regulated by the transmembrane signal transduction complex CnrYXH. Together, the metal sensor CnrX and the transmembrane antisigma factor CnrY control the availability of the extracytoplasmic function sigma factor CnrH. Release of CnrH from sequestration by CnrY at the cytoplasmic side of the membrane depends essentially on the binding of the agonist metal ion Ni(ii) to the periplasmic metal sensor domain of CnrX. CnrH availability leads to transcription initiation at the promoters cnrYp and cnrCp and to the expression of the genes in the cnrYXHCBA nickel resistance determinant. The first steps of signal propagation by CnrX rely on subtle metal-dependent allosteric modifications. To study the nickel-mediated triggering process by CnrX, we have altered selected residues, F66, M123, and Y135, and explored the physiological consequences of these changes with respect to metal resistance, expression of a cnrCBA-lacZ reporter fusion and protein production. M123C- and Y135F-CnrXs have been further characterized in vitro by metal affinity measurements and crystallographic structure analysis. Atomic-resolution structures of metal-bound M123C- and Y135F-CnrXs showed that Ni(ii) binds two of the three canonical conformations identified and that Ni(ii) sensing likely proceeds by conformation selection.
Response of CnrX from Cupriavidus metallidurans CH34 to nickel binding.,Maillard AP, Kunnemann S, Grosse C, Volbeda A, Schleuder G, Petit-Hartlein I, de Rosny E, Nies DH, Coves J Metallomics. 2015 Jan 28. PMID:25628016[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Grass G, Grosse C, Nies DH. Regulation of the cnr cobalt and nickel resistance determinant from Ralstonia sp. strain CH34. J Bacteriol. 2000 Mar;182(5):1390-8. PMID:10671463
- ↑ Tibazarwa C, Wuertz S, Mergeay M, Wyns L, van Der Lelie D. Regulation of the cnr cobalt and nickel resistance determinant of Ralstonia eutropha (Alcaligenes eutrophus) CH34. J Bacteriol. 2000 Mar;182(5):1399-409. PMID:10671464
- ↑ Maillard AP, Kunnemann S, Grosse C, Volbeda A, Schleuder G, Petit-Hartlein I, de Rosny E, Nies DH, Coves J. Response of CnrX from Cupriavidus metallidurans CH34 to nickel binding. Metallomics. 2015 Jan 28. PMID:25628016 doi:http://dx.doi.org/10.1039/c4mt00293h
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