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| | ==High-resolution NMR structures of the domains of Saccharomyces cerevisiae Tho1== | | ==High-resolution NMR structures of the domains of Saccharomyces cerevisiae Tho1== |
| - | <StructureSection load='4uzw' size='340' side='right'caption='[[4uzw]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='4uzw' size='340' side='right'caption='[[4uzw]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4uzw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UZW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UZW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4uzw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UZW FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uzm|4uzm]], [[4uzx|4uzx]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uzw OCA], [http://pdbe.org/4uzw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uzw RCSB], [http://www.ebi.ac.uk/pdbsum/4uzw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4uzw ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uzw OCA], [https://pdbe.org/4uzw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uzw RCSB], [https://www.ebi.ac.uk/pdbsum/4uzw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uzw ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/THO1_YEAST THO1_YEAST]] Unknown; suppressor of the transcriptional defect of HPR1 by overexpression. | + | [https://www.uniprot.org/uniprot/THO1_YEAST THO1_YEAST] Unknown; suppressor of the transcriptional defect of HPR1 by overexpression. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Allen, M D]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Bycroft, M]] | + | [[Category: Allen MD]] |
| - | [[Category: Freund, S M.V]] | + | [[Category: Bycroft M]] |
| - | [[Category: Jacobsen, J O.B]] | + | [[Category: Freund SMV]] |
| - | [[Category: Rna binding protein]] | + | [[Category: Jacobsen JOB]] |
| - | [[Category: Sap]]
| + | |
| Structural highlights
Function
THO1_YEAST Unknown; suppressor of the transcriptional defect of HPR1 by overexpression.
Publication Abstract from PubMed
THO is a multi-protein complex involved in the formation of messenger ribonuclear particles (mRNPs) by coupling transcription with mRNA processing and export. THO is thought to be formed from five subunits, Tho2p, Hpr1p, Tex1p, Mft1p and Thp2p, and recent work has determined a low-resolution structure of the complex [Poulsen et al. (2014), PLoS One, 9, e103470]. A number of additional proteins are thought to be involved in the formation of mRNP in yeast, including Tho1, which has been shown to bind RNA in vitro and is recruited to actively transcribed chromatin in vivo in a THO-complex and RNA-dependent manner. Tho1 is known to contain a SAP domain at the N-terminus, but the ability to suppress the expression defects of the hpr1Delta mutant of THO was shown to reside in the RNA-binding C-terminal region. In this study, high-resolution structures of both the N-terminal DNA-binding SAP domain and C-terminal RNA-binding domain have been determined.
High-resolution NMR structures of the domains of Saccharomyces cerevisiae Tho1.,Jacobsen JO, Allen MD, Freund SM, Bycroft M Acta Crystallogr F Struct Biol Commun. 2016 Jun 1;72(Pt 6):500-6. doi:, 10.1107/S2053230X16007597. Epub 2016 May 23. PMID:27303905[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jacobsen JO, Allen MD, Freund SM, Bycroft M. High-resolution NMR structures of the domains of Saccharomyces cerevisiae Tho1. Acta Crystallogr F Struct Biol Commun. 2016 Jun 1;72(Pt 6):500-6. doi:, 10.1107/S2053230X16007597. Epub 2016 May 23. PMID:27303905 doi:http://dx.doi.org/10.1107/S2053230X16007597
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