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| | <StructureSection load='4w4m' size='340' side='right'caption='[[4w4m]], [[Resolution|resolution]] 3.20Å' scene=''> | | <StructureSection load='4w4m' size='340' side='right'caption='[[4w4m]], [[Resolution|resolution]] 3.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4w4m]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Salty Salty]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W4M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W4M FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4w4m]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W4M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4W4M FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">prgK, STM2871 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 SALTY])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w4m OCA], [http://pdbe.org/4w4m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4w4m RCSB], [http://www.ebi.ac.uk/pdbsum/4w4m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4w4m ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4w4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w4m OCA], [https://pdbe.org/4w4m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4w4m RCSB], [https://www.ebi.ac.uk/pdbsum/4w4m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4w4m ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PRGK_SALTY PRGK_SALTY]] Required for invasion of epithelial cells. Could be involved in protein secretion. | + | [https://www.uniprot.org/uniprot/PRGK_SALTY PRGK_SALTY] Required for invasion of epithelial cells. Could be involved in protein secretion. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Salty]] | + | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]] |
| - | [[Category: Bergeron, J R.C]] | + | [[Category: Bergeron JRC]] |
| - | [[Category: Strynadka, N C.J]] | + | [[Category: Strynadka NCJ]] |
| - | [[Category: Protein transport]]
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| - | [[Category: Salmonella]]
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| - | [[Category: T3ss]]
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| Structural highlights
Function
PRGK_SALTY Required for invasion of epithelial cells. Could be involved in protein secretion.
Publication Abstract from PubMed
The type III secretion system (T3SS) is a large macromolecular assembly found at the surface of many pathogenic Gram-negative bacteria. Its role is to inject toxic "effector" proteins into the cells of infected organisms. The molecular details of the assembly of this large, multimembrane-spanning complex remain poorly understood. Here, we report structural, biochemical, and functional analyses of PrgK, an inner-membrane component of the prototypical Salmonella typhimurium T3SS. We have obtained the atomic structures of the two ring building globular domains and show that the C-terminal transmembrane helix is not essential for assembly and secretion. We also demonstrate that structural rearrangement of the two PrgK globular domains, driven by an interconnecting linker region, may promote oligomerization into ring structures. Finally, we used electron microscopy-guided symmetry modeling to propose a structural model for the intimately associated PrgH-PrgK ring interaction within the assembled basal body.
The Modular Structure of the Inner-Membrane Ring Component PrgK Facilitates Assembly of the Type III Secretion System Basal Body.,Bergeron JR, Worrall LJ, De S, Sgourakis NG, Cheung AH, Lameignere E, Okon M, Wasney GA, Baker D, McIntosh LP, Strynadka NC Structure. 2015 Jan 6;23(1):161-72. doi: 10.1016/j.str.2014.10.021. Epub 2014 Dec, 18. PMID:25533490[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bergeron JR, Worrall LJ, De S, Sgourakis NG, Cheung AH, Lameignere E, Okon M, Wasney GA, Baker D, McIntosh LP, Strynadka NC. The Modular Structure of the Inner-Membrane Ring Component PrgK Facilitates Assembly of the Type III Secretion System Basal Body. Structure. 2015 Jan 6;23(1):161-72. doi: 10.1016/j.str.2014.10.021. Epub 2014 Dec, 18. PMID:25533490 doi:http://dx.doi.org/10.1016/j.str.2014.10.021
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