2qco

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==Crystal structure of the transcriptional regulator CmeR from Campylobacter jejuni==
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The line below this paragraph, containing "STRUCTURE_2qco", creates the "Structure Box" on the page.
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<StructureSection load='2qco' size='340' side='right'caption='[[2qco]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2qco]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QCO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_2qco| PDB=2qco | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qco OCA], [https://pdbe.org/2qco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qco RCSB], [https://www.ebi.ac.uk/pdbsum/2qco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qco ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of the transcriptional regulator CmeR from Campylobacter jejuni'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q7B8P6_CAMJU Q7B8P6_CAMJU]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The CmeABC multidrug efflux pump, which belongs to the resistance-nodulation-division (RND) family, recognizes and extrudes a broad range of antimicrobial agents and is essential for Campylobacter jejuni colonization of the animal intestinal tract by mediating the efflux of bile acids. The expression of CmeABC is controlled by the transcriptional regulator CmeR, whose open reading frame is located immediately upstream of the cmeABC operon. To understand the structural basis of CmeR regulation, we have determined the crystal structure of CmeR to 2.2 A resolution, revealing a dimeric two-domain molecule with an entirely helical architecture similar to members of the TetR family of transcriptional regulators. Unlike the rest of the TetR regulators, CmeR has a large center-to-center distance (54 A) between two N termini of the dimer, and a large flexible ligand-binding pocket in the C-terminal domain. Each monomer forms a 20 A long tunnel-like cavity in the ligand-binding domain of CmeR and is occupied by a fortuitous ligand that is identified as glycerol. The binding of glycerol to CmeR induces a conformational state that is incompatible with target DNA. As glycerol has a chemical structure similar to that of potential ligands of CmeR, the structure obtained mimics the induced form of CmeR. These findings reveal novel structural features of a TetR family regulator, and provide new insight into the mechanisms of ligand binding and CmeR regulation.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qc/2qco_consurf.spt"</scriptWhenChecked>
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2QCO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QCO OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of the transcriptional regulator CmeR from Campylobacter jejuni., Gu R, Su CC, Shi F, Li M, McDermott G, Zhang Q, Yu EW, J Mol Biol. 2007 Sep 21;372(3):583-93. Epub 2007 Jul 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17686491 17686491]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qco ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Campylobacter jejuni]]
[[Category: Campylobacter jejuni]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Gu, R.]]
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[[Category: Gu R]]
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[[Category: Li, M.]]
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[[Category: Li M]]
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[[Category: McDermott, G.]]
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[[Category: McDermott G]]
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[[Category: Shi, F.]]
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[[Category: Shi F]]
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[[Category: Su, C.]]
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[[Category: Su C]]
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[[Category: Yu, E W.]]
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[[Category: Yu EW]]
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[[Category: Zhang, Q.]]
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[[Category: Zhang Q]]
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[[Category: Transcriptional regulator protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 16 23:05:09 2008''
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Current revision

Crystal structure of the transcriptional regulator CmeR from Campylobacter jejuni

PDB ID 2qco

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