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6rim

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Crystal structure of the catalytic domain of the Weissela oryzae botulinum like toxin

Structural highlights

6rim is a 8 chain structure with sequence from Weissella oryzae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.6Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BXWO_WEIOS When overexpressed the N-terminus (residues 1-476) cleaves rat synaptobrevin-2/VAMP2 between '89-Trp-|-Trp-90' in vitro. This releases the cytoplasmic domain of VAMP2 from the synaptic vesicle membrane, which would prevent the assembly of the trans-SNARE complex on the membrane and thus prevent vesicle-target membrane fusion and neurotransmitter release (PubMed:27443638).^[1]

Publication Abstract from PubMed

Botulinum neurotoxins (BoNTs) are the most potent toxins known. So far, eight serotypes have been identified that all act as zinc-dependent endopeptidases targeting SNARE proteins and inhibiting the release of neurotransmitters. Recently, the first botulinum toxin-like protein was identified outside the Clostridial genus, designated BoNT/Wo in the genome of Weissella oryzae. Here, we report the 1.6 A X-ray crystal structure of the light chain of BoNT/Wo (LC/Wo). LC/Wo presents the core fold common to BoNTs but has an unusually wide, open, and negatively charged catalytic pocket, with an additional Ca(2+) ion besides the zinc ion and a unique ss-hairpin motif. The structural information will help establish the substrate profile of BoNT/Wo and help our understanding of how BoNT evolved. This article is protected by copyright. All rights reserved.

Crystal structure of the catalytic domain of the Weissela oryzae botulinum-like toxin.,Kosenina S, Masuyer G, Zhang S, Dong M, Stenmark P FEBS Lett. 2019 May 20. doi: 10.1002/1873-3468.13446. PMID:31111466^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zornetta I, Azarnia Tehran D, Arrigoni G, Anniballi F, Bano L, Leka O, Zanotti G, Binz T, Montecucco C. The first non Clostridial botulinum-like toxin cleaves VAMP within the juxtamembrane domain. Sci Rep. 2016 Jul 22;6:30257. doi: 10.1038/srep30257. PMID:27443638 doi:http://dx.doi.org/10.1038/srep30257
↑ Kosenina S, Masuyer G, Zhang S, Dong M, Stenmark P. Crystal structure of the catalytic domain of the Weissela oryzae botulinum-like toxin. FEBS Lett. 2019 May 20. doi: 10.1002/1873-3468.13446. PMID:31111466 doi:http://dx.doi.org/10.1002/1873-3468.13446

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6rim"

Categories: Large Structures | Weissella oryzae | Kosenina S | Masuyer G | Stenmark P

@@ Line 3: / Line 3: @@
 <StructureSection load='6rim' size='340' side='right'caption='[[6rim]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6rim]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RIM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6rim]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Weissella_oryzae Weissella oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RIM FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rim OCA], [https://pdbe.org/6rim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rim RCSB], [https://www.ebi.ac.uk/pdbsum/6rim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rim ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rim OCA], [http://pdbe.org/6rim PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rim RCSB], [http://www.ebi.ac.uk/pdbsum/6rim PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rim ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/BXWO_WEIOS BXWO_WEIOS]] When overexpressed the N-terminus (residues 1-476) cleaves rat synaptobrevin-2/VAMP2 between '89-Trp-|-Trp-90' in vitro. This releases the cytoplasmic domain of VAMP2 from the synaptic vesicle membrane, which would prevent the assembly of the trans-SNARE complex on the membrane and thus prevent vesicle-target membrane fusion and neurotransmitter release (PubMed:27443638).<ref>PMID:27443638</ref>
+[https://www.uniprot.org/uniprot/BXWO_WEIOS BXWO_WEIOS] When overexpressed the N-terminus (residues 1-476) cleaves rat synaptobrevin-2/VAMP2 between '89-Trp-|-Trp-90' in vitro. This releases the cytoplasmic domain of VAMP2 from the synaptic vesicle membrane, which would prevent the assembly of the trans-SNARE complex on the membrane and thus prevent vesicle-target membrane fusion and neurotransmitter release (PubMed:27443638).<ref>PMID:27443638</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Bontoxilysin]]
 [[Category: Large Structures]]
-[[Category: Kosenina, S]]
+[[Category: Weissella oryzae]]
-[[Category: Masuyer, G]]
+[[Category: Kosenina S]]
-[[Category: Stenmark, P]]
+[[Category: Masuyer G]]
-[[Category: Botulinum neurotoxin]]
+[[Category: Stenmark P]]
-[[Category: Toxin]]
-[[Category: Zinc endopeptidase]]

6rim

From Proteopedia

Current revision

Crystal structure of the catalytic domain of the Weissela oryzae botulinum like toxin

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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