2ioy

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[[Image:2ioy.jpg|left|200px]]
 
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==Crystal structure of Thermoanaerobacter tengcongensis ribose binding protein==
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The line below this paragraph, containing "STRUCTURE_2ioy", creates the "Structure Box" on the page.
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<StructureSection load='2ioy' size='340' side='right'caption='[[2ioy]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2ioy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IOY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IOY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RIP:RIBOSE(PYRANOSE+FORM)'>RIP</scene></td></tr>
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{{STRUCTURE_2ioy| PDB=2ioy | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ioy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ioy OCA], [https://pdbe.org/2ioy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ioy RCSB], [https://www.ebi.ac.uk/pdbsum/2ioy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ioy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8RD41_CALS4 Q8RD41_CALS4]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/io/2ioy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ioy ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of Thermoanaerobacter tengcongensis ribose binding protein'''
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==See Also==
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*[[Ribose-binding protein|Ribose-binding protein]]
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__TOC__
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==Overview==
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</StructureSection>
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ABSTRACT: BACKGROUND: Comparison of experimentally determined mesophilic and thermophilic homologous protein structures is an important tool for understanding the mechanisms that contribute to thermal stability. Of particular interest are pairs of homologous structures that are structurally very similar, but differ significantly in thermal stability. RESULTS: We report the X-ray crystal structure of a Thermoanaerobacter tengcongensis ribose binding protein (tteRBP) determined to 1.9A resolution. We find that tteRBP is significantly more stable (appTm value ~102AdegreesC) than the mesophilic Escherichia coli ribose binding protein (ecRBP) (appTm value ~56AdegreesC). The tteRBP has essentially the identical backbone conformation (0.41A RMSD of 235/271 C-alpha positions and 0.65A RMSD of 270/271 C-alpha positions) as ecRBP. Classification of the amino acid substitutions as a function of structure therefore allows the identification of amino acids which potentially contribute to the observed thermal stability of tteRBP in the absence of large structural heterogeneities. CONCLUSIONS: The near identity of backbone structures of this pair of proteins entails that the significant differences in their thermal stabilities are encoded exclusively by the identity of the amino acid side-chains. Furthermore, the degree of sequence divergence is strongly correlated with structure; with a high degree of conservation in the core progressing to increased diversity in the boundary and surface regions. Different factors that may possibly contribute to thermal stability appear to be differentially encoded in each of these regions of the protein. The tteRBP/ecRBP pair therefore offers an opportunity to dissect contributions to thermal stability by side-chains alone in the absence of large structural differences.
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[[Category: Caldanaerobacter subterraneus subsp. tengcongensis]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Cuneo MJ]]
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2IOY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacter_tengcongensis Thermoanaerobacter tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IOY OCA].
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[[Category: Hellinga HW]]
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==Reference==
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The backbone structure of the thermophilic Thermoanaerobacter tengcongensis ribose binding protein is essentially identical to its mesophilic E. coli homolog., Cuneo MJ, Tian Y, Allert M, Hellinga HW, BMC Struct Biol. 2008 Mar 28;8(1):20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18373848 18373848]
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[[Category: Single protein]]
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[[Category: Thermoanaerobacter tengcongensis]]
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[[Category: Cuneo, M J.]]
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[[Category: Hellinga, H W.]]
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[[Category: Ribose binding protein]]
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[[Category: Sugar binding protein]]
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[[Category: Thermophilic protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 16 23:06:41 2008''
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Current revision

Crystal structure of Thermoanaerobacter tengcongensis ribose binding protein

PDB ID 2ioy

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