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| | <StructureSection load='4xau' size='340' side='right'caption='[[4xau]], [[Resolution|resolution]] 3.00Å' scene=''> | | <StructureSection load='4xau' size='340' side='right'caption='[[4xau]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4xau]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/'actinomadura_melliaura' 'actinomadura melliaura']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XAU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XAU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4xau]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinomadura_melliaura Actinomadura melliaura]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XAU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XAU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.0012Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4rxk|4rxk]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">atS13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=360723 'Actinomadura melliaura'])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xau OCA], [https://pdbe.org/4xau PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xau RCSB], [https://www.ebi.ac.uk/pdbsum/4xau PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xau ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xau OCA], [http://pdbe.org/4xau PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xau RCSB], [http://www.ebi.ac.uk/pdbsum/4xau PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xau ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q0H2X1_9ACTN Q0H2X1_9ACTN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Actinomadura melliaura]] | | [[Category: Actinomadura melliaura]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis]]
| + | [[Category: Phillips Jr GN]] |
| - | [[Category: Phillips, G N]] | + | [[Category: Singh S]] |
| - | [[Category: Singh, S]] | + | [[Category: Thorson JS]] |
| - | [[Category: Thorson, J S]] | + | [[Category: Wang F]] |
| - | [[Category: Wang, F]] | + | [[Category: Xu W]] |
| - | [[Category: Xu, W]] | + | |
| - | [[Category: Enzyme discovery for natural product biosynthesis]]
| + | |
| - | [[Category: Natpro]]
| + | |
| - | [[Category: PSI, Protein structure initiative]]
| + | |
| - | [[Category: Structural genomic]]
| + | |
| - | [[Category: Sugar aminotransferase]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
Q0H2X1_9ACTN
Publication Abstract from PubMed
AT2433 from Actinomadura melliaura is an indolocarbazole antitumor antibiotic structurally distinguished by its unique aminodideoxypentose-containing disaccharide moiety. The corresponding sugar nucleotide-based biosynthetic pathway for this unusual sugar derives from comparative genomics where AtmS13 has been suggested as the contributing sugar aminotransferase (SAT). Determination of the AtmS13 X-ray structure at 1.50 A resolution reveals it as a member of the aspartate aminotransferase fold type I (AAT-I). Structural comparisons of AtmS13 with homologous SATs that act upon similar substrates implicate potential active site residues that contribute to distinctions in sugar C5 (hexose versus pentose) and/or sugar C2 (deoxy versus hydroxyl) substrate specificity. This article is protected by copyright. All rights reserved.
Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis.,Singh S, Kim Y, Wang F, Bigelow L, Endres M, Kharel MK, Babnigg G, Bingman CA, Joachimiak A, Thorson JS, Phillips GN Proteins. 2015 Jun 9. doi: 10.1002/prot.24844. PMID:26061967[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Singh S, Kim Y, Wang F, Bigelow L, Endres M, Kharel MK, Babnigg G, Bingman CA, Joachimiak A, Thorson JS, Phillips GN. Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis. Proteins. 2015 Jun 9. doi: 10.1002/prot.24844. PMID:26061967 doi:http://dx.doi.org/10.1002/prot.24844
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