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4y68

From Proteopedia

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Structure of a lipoprotein from Streptococcus agalactiae

Structural highlights

4y68 is a 4 chain structure with sequence from Streptococcus agalactiae 515. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.21Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A140UHB6_STRAG

Publication Abstract from PubMed

Lantibiotics are potent antimicrobial peptides. Nisin is the most prominent member and contains five crucial lanthionine rings. Some clinically relevant bacteria express membrane-associated resistance proteins that proteolytically inactivate nisin. However, substrate recognition and specificity of these proteins is unknown. Here, we report the first three-dimensional structure of a nisin resistance protein from Streptococcus agalactiae (SaNSR) at 2.2 A resolution. It contains an N-terminal helical bundle, and protease cap and core domains. The latter harbors the highly conserved TASSAEM region, which lies in a hydrophobic tunnel formed by all domains. By integrative modeling, mutagenesis studies, and genetic engineering of nisin variants, a model of the SaNSR/nisin complex is generated, revealing that SaNSR recognizes the last C-terminally located lanthionine ring of nisin. This determines the substrate specificity of SaNSR and ensures the exact coordination of the nisin cleavage site at the TASSAEM region.

Structural basis of lantibiotic recognition by the nisin resistance protein from Streptococcus agalactiae.,Khosa S, Frieg B, Mulnaes D, Kleinschrodt D, Hoeppner A, Gohlke H, Smits SH Sci Rep. 2016 Jan 4;6:18679. doi: 10.1038/srep18679. PMID:26727488^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Khosa S, Frieg B, Mulnaes D, Kleinschrodt D, Hoeppner A, Gohlke H, Smits SH. Structural basis of lantibiotic recognition by the nisin resistance protein from Streptococcus agalactiae. Sci Rep. 2016 Jan 4;6:18679. doi: 10.1038/srep18679. PMID:26727488 doi:http://dx.doi.org/10.1038/srep18679

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4y68"

Categories: Large Structures | Streptococcus agalactiae 515 | Hoeppner A | Khosa S | Smits SH

@@ Line 3: / Line 3: @@
 <StructureSection load='4y68' size='340' side='right'caption='[[4y68]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4y68]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_515 Streptococcus agalactiae 515]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y68 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4y68]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_agalactiae_515 Streptococcus agalactiae 515]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y68 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.21&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SAL_1042 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=342614 Streptococcus agalactiae 515])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y68 OCA], [http://pdbe.org/4y68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y68 RCSB], [http://www.ebi.ac.uk/pdbsum/4y68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4y68 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y68 OCA], [https://pdbe.org/4y68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y68 RCSB], [https://www.ebi.ac.uk/pdbsum/4y68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y68 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A140UHB6_STRAG A0A140UHB6_STRAG]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 25: @@
 [[Category: Large Structures]]
 [[Category: Streptococcus agalactiae 515]]
-[[Category: Hoeppner, A]]
+[[Category: Hoeppner A]]
-[[Category: Khosa, S]]
+[[Category: Khosa S]]
-[[Category: Smits, S H]]
+[[Category: Smits SH]]
-[[Category: Hydrolase]]
-[[Category: Lantibiotic]]
-[[Category: Lipoprotein]]
-[[Category: Peptidase]]

4y68

From Proteopedia

Current revision

Structure of a lipoprotein from Streptococcus agalactiae

Structural highlights

Function

Publication Abstract from PubMed

References

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