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| | ==SOLUTION STRUCTURE OF BMP02, A NATURAL SCORPION TOXIN WHICH BLOCKS APAMIN-SENSITIVE CALCIUM-ACTIVATED POTASSIUM CHANNELS, 25 STRUCTURES== | | ==SOLUTION STRUCTURE OF BMP02, A NATURAL SCORPION TOXIN WHICH BLOCKS APAMIN-SENSITIVE CALCIUM-ACTIVATED POTASSIUM CHANNELS, 25 STRUCTURES== |
| - | <StructureSection load='1du9' size='340' side='right'caption='[[1du9]], [[NMR_Ensembles_of_Models | 25 NMR models]]' scene=''> | + | <StructureSection load='1du9' size='340' side='right'caption='[[1du9]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1du9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DU9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DU9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1du9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DU9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DU9 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1du9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1du9 OCA], [http://pdbe.org/1du9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1du9 RCSB], [http://www.ebi.ac.uk/pdbsum/1du9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1du9 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 25 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1du9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1du9 OCA], [https://pdbe.org/1du9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1du9 RCSB], [https://www.ebi.ac.uk/pdbsum/1du9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1du9 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/KAX91_MESMA KAX91_MESMA]] Blocks small conductance calcium-activated potassium channels (KCNN, SK). Weakly inhibits the Kv7.1/KCNQ1 channel (10 uM of the toxin inhibits currents by 23.3%). Low toxicity by intracerebroventricular injection into mice.<ref>PMID:22511981</ref> | + | [https://www.uniprot.org/uniprot/KAX91_MESMA KAX91_MESMA] Blocks small conductance calcium-activated potassium channels (KCNN, SK). Weakly inhibits the Kv7.1/KCNQ1 channel (10 uM of the toxin inhibits currents by 23.3%). Low toxicity by intracerebroventricular injection into mice.<ref>PMID:22511981</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Potassium channel toxin|Potassium channel toxin]] | + | *[[Potassium channel toxin 3D structures|Potassium channel toxin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Mesobuthus martensii]] | | [[Category: Mesobuthus martensii]] |
| - | [[Category: Ji, Y]] | + | [[Category: Ji Y]] |
| - | [[Category: Pei, J]] | + | [[Category: Pei J]] |
| - | [[Category: Shi, Y]] | + | [[Category: Shi Y]] |
| - | [[Category: Tong, Q]] | + | [[Category: Tong Q]] |
| - | [[Category: Wu, J]] | + | [[Category: Wu J]] |
| - | [[Category: Xu, Y]] | + | [[Category: Xu Y]] |
| - | [[Category: Helix]]
| + | |
| - | [[Category: Sheet]]
| + | |
| - | [[Category: Toxin]]
| + | |
| Structural highlights
Function
KAX91_MESMA Blocks small conductance calcium-activated potassium channels (KCNN, SK). Weakly inhibits the Kv7.1/KCNQ1 channel (10 uM of the toxin inhibits currents by 23.3%). Low toxicity by intracerebroventricular injection into mice.[1]
Publication Abstract from PubMed
BmP02 is a 28-amino acid residue peptide purified from the venom of the Chinese scorpion Buthus martensi Karsch, which had been demonstrated to be a weak blocker of apamin-sensitive calcium-activated potassium channels. Two-dimensional NMR spectroscopy techniques were used to determine the solution structure of BmP02. The results show that BmP02 formed a alpha/beta scorpion fold, the typical three-dimensional structure adopted by most short chain scorpion toxins whose structures have been determined. However, in BmP02 this alpha/beta fold was largely distorted. The alpha-helix was shortened to only one turn, and the loop connecting the helix to the first beta-strand exhibited conformational heterogeneity. The instability of BmP02 could be attributed to a proline at position 17, which is usually a glycine. Because the residue at this position makes intense contact with the alpha-helix, it was supposed that the bulky side chain of proline had pushed the helix away from the beta-sheet. This had a significant influence on the structure and function of BmP02. The alpha-helix rotated by about 40 degrees to avoid Pro17 while forming two disulfides with the second beta-strand. The rotation further caused both ends of the helix to be unwound due to covalent restrictions. According to its structure, BmP02 was supposed to interact with its target via the side chains of Lys11 and Lys13.
Solution structure of BmP02, a new potassium channel blocker from the venom of the Chinese scorpion Buthus martensi Karsch.,Xu Y, Wu J, Pei J, Shi Y, Ji Y, Tong Q Biochemistry. 2000 Nov 14;39(45):13669-75. PMID:11076505[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Chen ZY, Zeng DY, Hu YT, He YW, Pan N, Ding JP, Cao ZJ, Liu ML, Li WX, Yi H, Jiang L, Wu YL. Structural and functional diversity of acidic scorpion potassium channel toxins. PLoS One. 2012;7(4):e35154. Epub 2012 Apr 12. PMID:22511981 doi:10.1371/journal.pone.0035154
- ↑ Xu Y, Wu J, Pei J, Shi Y, Ji Y, Tong Q. Solution structure of BmP02, a new potassium channel blocker from the venom of the Chinese scorpion Buthus martensi Karsch. Biochemistry. 2000 Nov 14;39(45):13669-75. PMID:11076505
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