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| | <StructureSection load='4y7i' size='340' side='right'caption='[[4y7i]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='4y7i' size='340' side='right'caption='[[4y7i]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4y7i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y7I FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4y7i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y7I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y7I FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.802Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MTMR8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y7i OCA], [http://pdbe.org/4y7i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y7i RCSB], [http://www.ebi.ac.uk/pdbsum/4y7i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4y7i ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y7i OCA], [https://pdbe.org/4y7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y7i RCSB], [https://www.ebi.ac.uk/pdbsum/4y7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y7i ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MTMR8_HUMAN MTMR8_HUMAN]] Phosphatase that acts on lipids with a phosphoinositol headgroup. | + | [https://www.uniprot.org/uniprot/MTMR8_HUMAN MTMR8_HUMAN] Phosphatase that acts on lipids with a phosphoinositol headgroup. |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
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| - | Myotubularin-related proteins are a large family of phosphoinositide phosphatases; their activity, stability and subcellular localization are regulated by dimeric interactions with other members of the family. Here, the crystal structure of the phosphatase domain of MTMR8 is reported. Conformational deviation of the two loops that mediate interaction with the PH-GRAM domain suggests that the PH-GRAM domain interacts differently with the phosphatase domain of each MTMR member. The protein exists as a dimer with twofold symmetry, providing insight into a novel mode of dimerization mediated by the phosphatase domain. Structural comparison and mutation studies suggest that Lys255 of MTMR8 interacts with the substrate diacylglycerol moiety, similar to Lys333 of MTMR2, although the positions of these residues are different. The catalytic activity of the MTMR8 phosphatase domain is inhibited by oxidation and is reversibly reactivated by reduction, suggesting the presence of an oxidation-protective intermediate other than a disulfide bond owing to the absence of a cysteine within a disulfide-bond distance from Cys338.
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| - | Structure of the catalytic phosphatase domain of MTMR8: implications for dimerization, membrane association and reversible oxidation.,Yoo KY, Son JY, Lee JU, Shin W, Im DW, Kim SJ, Ryu SE, Heo YS Acta Crystallogr D Biol Crystallogr. 2015 Jul 1;71(Pt 7):1528-39. doi:, 10.1107/S139900471500927X. Epub 2015 Jun 30. PMID:26143924<ref>PMID:26143924</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 4y7i" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Heo, Y S]] | + | [[Category: Heo YS]] |
| - | [[Category: Im, D]] | + | [[Category: Im D]] |
| - | [[Category: Lee, J]] | + | [[Category: Lee J]] |
| - | [[Category: Shin, W]] | + | [[Category: Shin W]] |
| - | [[Category: Son, J]] | + | [[Category: Son J]] |
| - | [[Category: Yoo, K]] | + | [[Category: Yoo K]] |
| - | [[Category: Hydrolase]]
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| - | [[Category: Mtmr8]]
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| - | [[Category: Myotubularin related protein]]
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| - | [[Category: Phosphatase]]
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| - | [[Category: Phosphoinositid]]
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