5o6b
From Proteopedia
(Difference between revisions)
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<StructureSection load='5o6b' size='340' side='right'caption='[[5o6b]], [[Resolution|resolution]] 2.03Å' scene=''> | <StructureSection load='5o6b' size='340' side='right'caption='[[5o6b]], [[Resolution|resolution]] 2.03Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5o6b]] is a 4 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[5o6b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O6B FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.029Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o6b OCA], [https://pdbe.org/5o6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o6b RCSB], [https://www.ebi.ac.uk/pdbsum/5o6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o6b ProSAT]</span></td></tr> | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/PIF1_YEAST PIF1_YEAST] DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Appears to move along DNA in single nucleotide or base pair steps, powered by hydrolysis of 1 molecule of ATP. Processes at an unwinding rate of about 75 bp/s. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Involved in the maintenance of ribosomal (rDNA). Required for efficient fork arrest at the replication fork barrier within rDNA. Involved in the maintenance of mitochondrial (mtDNA). Required to maintain mtDNA under conditions that introduce dsDNA breaks in mtDNA, either preventing or repairing dsDNA breaks. May inhibit replication progression to allow time for repair. May have a general role in chromosomal replication by affecting Okazaki fragment maturation. May have a role in conjunction with DNA2 helicase/nuclease in 5'-flap extension during Okazaki fragment processing.[HAMAP-Rule:MF_03176]<ref>PMID:10693764</ref> <ref>PMID:10926538</ref> <ref>PMID:11429610</ref> <ref>PMID:12024022</ref> <ref>PMID:15907372</ref> <ref>PMID:15923634</ref> <ref>PMID:16121131</ref> <ref>PMID:16537895</ref> <ref>PMID:16816432</ref> <ref>PMID:16878131</ref> <ref>PMID:17257907</ref> <ref>PMID:17590086</ref> <ref>PMID:17720711</ref> <ref>PMID:1849081</ref> <ref>PMID:19424434</ref> <ref>PMID:20225162</ref> <ref>PMID:21620135</ref> <ref>PMID:23175612</ref> <ref>PMID:23446274</ref> <ref>PMID:23596008</ref> <ref>PMID:23657261</ref> <ref>PMID:3038524</ref> <ref>PMID:8287473</ref> |
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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==See Also== | ==See Also== | ||
| - | *[[Helicase|Helicase]] | + | *[[Helicase 3D structures|Helicase 3D structures]] |
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Baker's yeast]] | ||
| - | [[Category: DNA helicase]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: | + | [[Category: Chen WF]] |
| - | [[Category: | + | [[Category: Liu NN]] |
| - | [[Category: | + | [[Category: Lu KY]] |
| - | [[Category: | + | [[Category: Rety S]] |
| - | [[Category: | + | [[Category: Xu XG]] |
Current revision
Structure of ScPif1 in complex with GGGTTTT and ADP-AlF4
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