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| | ==PHEROMONE ER-11, NMR== | | ==PHEROMONE ER-11, NMR== |
| - | <StructureSection load='1ery' size='340' side='right'caption='[[1ery]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1ery' size='340' side='right'caption='[[1ery]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1ery]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Euplotes_raikovi Euplotes raikovi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ERY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ERY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1ery]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Euplotes_raikovi Euplotes raikovi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ERY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ERY FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ery FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ery OCA], [http://pdbe.org/1ery PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ery RCSB], [http://www.ebi.ac.uk/pdbsum/1ery PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ery ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ery FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ery OCA], [https://pdbe.org/1ery PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ery RCSB], [https://www.ebi.ac.uk/pdbsum/1ery PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ery ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MER11_EUPRA MER11_EUPRA]] Mating ciliate pheromones (or gamones) are diffusible extracellular communication signals that distinguish different intraspecific classes of cells commonly referred to as 'mating types'. They prepare the latter for conjugation by changing their cell surface properties. | + | [https://www.uniprot.org/uniprot/MER11_EUPRA MER11_EUPRA] Mating ciliate pheromones (or gamones) are diffusible extracellular communication signals that distinguish different intraspecific classes of cells commonly referred to as 'mating types'. They prepare the latter for conjugation by changing their cell surface properties. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Euplotes raikovi]] | | [[Category: Euplotes raikovi]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Luginbuhl, P]] | + | [[Category: Luginbuhl P]] |
| - | [[Category: Luporini, P]] | + | [[Category: Luporini P]] |
| - | [[Category: Ortenzi, C]] | + | [[Category: Ortenzi C]] |
| - | [[Category: Wu, J]] | + | [[Category: Wu J]] |
| - | [[Category: Wuthrich, K]] | + | [[Category: Wuthrich K]] |
| - | [[Category: Zerbe, O]] | + | [[Category: Zerbe O]] |
| - | [[Category: Pheromone]]
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| Structural highlights
Function
MER11_EUPRA Mating ciliate pheromones (or gamones) are diffusible extracellular communication signals that distinguish different intraspecific classes of cells commonly referred to as 'mating types'. They prepare the latter for conjugation by changing their cell surface properties.
Publication Abstract from PubMed
The NMR solution structure of the pheromone Er-11, a 39-residue protein from the ciliated protozoan Euplotes raikovi, was calculated with the distance geometry program DIANA from 449 NOE upper distance constraints and 97 dihedral angle constraints, and the program OPAL was employed for structure refinement by molecular mechanics energy minimization in a water bath. For a group of 20 conformers used to characterize the solution structure, the average of the pairwise RMS deviations from the mean structure calculated for the backbone heavy atoms N, C alpha, and C' of residues 2-38 was 0.30 A. The molecular architecture is dominated by an up-down-up bundle of three short helices with residues 2-9, 12-19, and 22-32, which is closely similar to the previously determined structures of the homologous pheromones Er-1, Er-2, and Er-10. This finding provides structural evidence for the capability shown by these pheromones to compete with each other in binding reactions to their cell-surface receptors.
The NMR solution structure of the pheromone Er-11 from the ciliated protozoan Euplotes raikovi.,Luginbuhl P, Wu J, Zerbe O, Ortenzi C, Luporini P, Wuthrich K Protein Sci. 1996 Aug;5(8):1512-22. PMID:8844842[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Luginbuhl P, Wu J, Zerbe O, Ortenzi C, Luporini P, Wuthrich K. The NMR solution structure of the pheromone Er-11 from the ciliated protozoan Euplotes raikovi. Protein Sci. 1996 Aug;5(8):1512-22. PMID:8844842
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