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| | ==CONSERVED DOMAIN COMMON TO TRANSCRIPTION FACTORS TFIIS, ELONGIN A, CRSP70== | | ==CONSERVED DOMAIN COMMON TO TRANSCRIPTION FACTORS TFIIS, ELONGIN A, CRSP70== |
| - | <StructureSection load='1eo0' size='340' side='right'caption='[[1eo0]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='1eo0' size='340' side='right'caption='[[1eo0]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1eo0]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EO0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EO0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1eo0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EO0 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1enw|1enw]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eo0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eo0 OCA], [http://pdbe.org/1eo0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eo0 RCSB], [http://www.ebi.ac.uk/pdbsum/1eo0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1eo0 ProSAT], [http://www.topsan.org/Proteins/NESGC/1eo0 TOPSAN]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eo0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eo0 OCA], [https://pdbe.org/1eo0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eo0 RCSB], [https://www.ebi.ac.uk/pdbsum/1eo0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eo0 ProSAT], [https://www.topsan.org/Proteins/NESGC/1eo0 TOPSAN]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TFS2_YEAST TFS2_YEAST]] Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Can promote the transfer of one strand of a double-stranded DNA molecule to a homologous single strand and thus may be involved in recombination. | + | [https://www.uniprot.org/uniprot/TFS2_YEAST TFS2_YEAST] Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Can promote the transfer of one strand of a double-stranded DNA molecule to a homologous single strand and thus may be involved in recombination. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Elongation factor|Elongation factor]] | + | *[[Elongation factor 3D structures|Elongation factor 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Arrowsmith, C H]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Booth, V]] | + | [[Category: Arrowsmith CH]] |
| - | [[Category: Edwards, A M]] | + | [[Category: Booth V]] |
| - | [[Category: Koth, C]] | + | [[Category: Edwards AM]] |
| - | [[Category: Structural genomic]] | + | [[Category: Koth C]] |
| - | [[Category: Helix-bundle]]
| + | |
| - | [[Category: Nesg]]
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| - | [[Category: PSI, Protein structure initiative]]
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| - | [[Category: Transcription]]
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| Structural highlights
Function
TFS2_YEAST Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Can promote the transfer of one strand of a double-stranded DNA molecule to a homologous single strand and thus may be involved in recombination.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
TFIIS is a transcription elongation factor that consists of three domains. We have previously solved the structures of domains II and III, which stimulate arrested polymerase II elongation complexes in order to resume transcription. Domain I is conserved in evolution from yeast to human species and is homologous to the transcription factors elongin A and CRSP70. Domain I also interacts with the transcriptionally active RNA polymerase II holoenzyme and therefore, may have a function unrelated to the previously described transcription elongation activity of TFIIS. We have solved the structure of domain I of yeast TFIIS using NMR spectroscopy. Domain I is a compact four-helix bundle that is structurally independent of domains II and III of the TFIIS. Using the yeast structure as a template, we have modeled the homologous domains from elongin A and CRSP70 and identified a conserved positively charged patch on the surface of all three proteins, which may be involved in conserved functional interactions with the transcriptional machinery.
Structure of a conserved domain common to the transcription factors TFIIS, elongin A, and CRSP70.,Booth V, Koth CM, Edwards AM, Arrowsmith CH J Biol Chem. 2000 Oct 6;275(40):31266-8. PMID:10811649[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Booth V, Koth CM, Edwards AM, Arrowsmith CH. Structure of a conserved domain common to the transcription factors TFIIS, elongin A, and CRSP70. J Biol Chem. 2000 Oct 6;275(40):31266-8. PMID:10811649 doi:http://dx.doi.org/10.1074/jbc.M002595200
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