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| | ==SOLUTION STRUCTURE OF ESCHERICHIA COLI NUSB== | | ==SOLUTION STRUCTURE OF ESCHERICHIA COLI NUSB== |
| - | <StructureSection load='1ey1' size='340' side='right'caption='[[1ey1]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''> | + | <StructureSection load='1ey1' size='340' side='right'caption='[[1ey1]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1ey1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EY1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EY1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1ey1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EY1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EY1 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ey1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ey1 OCA], [http://pdbe.org/1ey1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ey1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ey1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ey1 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ey1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ey1 OCA], [https://pdbe.org/1ey1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ey1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ey1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ey1 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NUSB_ECOLI NUSB_ECOLI]] One of the proteins essential for the formation of the RNA polymerase antitermination complex in the presence of lambda phage N protein. However, it is involved in the transcription termination process at certain sites during normal bacterial growth. Binds to the BoxA RNA motif. | + | [https://www.uniprot.org/uniprot/NUSB_ECOLI NUSB_ECOLI] One of the proteins essential for the formation of the RNA polymerase antitermination complex in the presence of lambda phage N protein. However, it is involved in the transcription termination process at certain sites during normal bacterial growth. Binds to the BoxA RNA motif. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | [[Category: Escherichia coli]] | | [[Category: Escherichia coli]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Altieri, A S]] | + | [[Category: Altieri AS]] |
| - | [[Category: Coats, R H]] | + | [[Category: Byrd RA]] |
| - | [[Category: Horita, D A]] | + | [[Category: Coats RH]] |
| - | [[Category: Mazzulla, M J]] | + | [[Category: Horita DA]] |
| - | [[Category: Wingfield, P T]] | + | [[Category: Mazzulla MJ]] |
| - | [[Category: Transcirption termination]] | + | [[Category: Wingfield PT]] |
| - | [[Category: Transcription]]
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| Structural highlights
Function
NUSB_ECOLI One of the proteins essential for the formation of the RNA polymerase antitermination complex in the presence of lambda phage N protein. However, it is involved in the transcription termination process at certain sites during normal bacterial growth. Binds to the BoxA RNA motif.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
We have determined the solution structure of NusB, a transcription antitermination protein from Escherichia coli. The structure reveals a novel, all alpha-helical protein fold. NusB mutations that cause a loss of function (NusB5) or alter specificity for RNA targets (NusB101) are localized to surface residues and likely affect RNA-protein or protein-protein interactions. Residues that are highly conserved among homologs stabilize the protein core. The solution structure of E. coli NusB presented here resembles that of Mycobacterium tuberculosis NusB determined by X-ray diffraction, but differs substantially from a solution structure of E. coli NusB reported earlier.
The structure of the transcriptional antiterminator NusB from Escherichia coli.,Altieri AS, Mazzulla MJ, Horita DA, Coats RH, Wingfield PT, Das A, Court DL, Byrd RA Nat Struct Biol. 2000 Jun;7(6):470-4. PMID:10881193[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Altieri AS, Mazzulla MJ, Horita DA, Coats RH, Wingfield PT, Das A, Court DL, Byrd RA. The structure of the transcriptional antiterminator NusB from Escherichia coli. Nat Struct Biol. 2000 Jun;7(6):470-4. PMID:10881193 doi:10.1038/75869
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