1f5x

From Proteopedia

(Difference between revisions)

Current revision

NMR STRUCTURE OF THE Y174 AUTOINHIBITED DBL HOMOLOGY DOMAIN

Structural highlights

1f5x is a 1 chain structure with sequence from Mus musculus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VAV_MOUSE Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Rho-family GTPases transduce signals from receptors leading to changes in cell shape and motility, mitogenesis, and development. Proteins containing the Dbl homology (DH) domain are responsible for activating Rho GTPases by catalyzing the exchange of GDP for GTP. Receptor-initiated stimulation of Dbl protein Vav exchange activity involves tyrosine phosphorylation. We show through structure determination that the mVav1 DH domain is autoinhibited by an N-terminal extension, which lies in the GTPase interaction site. This extension contains the Tyr174 Src-family kinase recognition site, and phosphorylation or truncation of this peptide results in stimulation of GEF activity. NMR spectroscopy data show that the N-terminal peptide is released from the DH domain and becomes unstructured upon phosphorylation. Thus, tyrosine phosphorylation relieves autoinhibition by exposing the GTPase interaction surface of the DH domain, which is obligatory for Vav activation.

Structural basis for relief of autoinhibition of the Dbl homology domain of proto-oncogene Vav by tyrosine phosphorylation.,Aghazadeh B, Lowry WE, Huang XY, Rosen MK Cell. 2000 Sep 1;102(5):625-33. PMID:11007481^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Aghazadeh B, Lowry WE, Huang XY, Rosen MK. Structural basis for relief of autoinhibition of the Dbl homology domain of proto-oncogene Vav by tyrosine phosphorylation. Cell. 2000 Sep 1;102(5):625-33. PMID:11007481

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1f5x"

@@ Line 1: / Line 1: @@
 ==NMR STRUCTURE OF THE Y174 AUTOINHIBITED DBL HOMOLOGY DOMAIN==
-<StructureSection load='1f5x' size='340' side='right'caption='[[1f5x]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='1f5x' size='340' side='right'caption='[[1f5x]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1f5x]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F5X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F5X FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1f5x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F5X FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f5x OCA], [http://pdbe.org/1f5x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1f5x RCSB], [http://www.ebi.ac.uk/pdbsum/1f5x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1f5x ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f5x OCA], [https://pdbe.org/1f5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f5x RCSB], [https://www.ebi.ac.uk/pdbsum/1f5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f5x ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/VAV_MOUSE VAV_MOUSE]] Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
+[https://www.uniprot.org/uniprot/VAV_MOUSE VAV_MOUSE] Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 33: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Aghazadeh, B]]
+[[Category: Mus musculus]]
-[[Category: Huang, X Y]]
+[[Category: Aghazadeh B]]
-[[Category: Lowry, W E]]
+[[Category: Huang XY]]
-[[Category: Rosen, M K]]
+[[Category: Lowry WE]]
-[[Category: 11 alpha-helice]]
+[[Category: Rosen MK]]
-[[Category: Signaling protein]]

1f5x

From Proteopedia

Current revision

NMR STRUCTURE OF THE Y174 AUTOINHIBITED DBL HOMOLOGY DOMAIN

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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