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| | <StructureSection load='4yki' size='340' side='right'caption='[[4yki]], [[Resolution|resolution]] 1.21Å' scene=''> | | <StructureSection load='4yki' size='340' side='right'caption='[[4yki]], [[Resolution|resolution]] 1.21Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4yki]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/American_comb_jelly American comb jelly]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YKI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YKI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4yki]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mnemiopsis_leidyi Mnemiopsis leidyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YKI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YKI FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.21Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ykj|4ykj]], [[4ykk|4ykk]], [[4ykp|4ykp]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ML032222a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=27923 American comb jelly])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yki OCA], [https://pdbe.org/4yki PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yki RCSB], [https://www.ebi.ac.uk/pdbsum/4yki PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yki ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yki OCA], [http://pdbe.org/4yki PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yki RCSB], [http://www.ebi.ac.uk/pdbsum/4yki PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4yki ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0R4I973_MNELE A0A0R4I973_MNELE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: American comb jelly]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Alberstein, R G]] | + | [[Category: Mnemiopsis leidyi]] |
| - | [[Category: Mayer, M L]] | + | [[Category: Alberstein RG]] |
| - | [[Category: Glutamate receptor]] | + | [[Category: Mayer ML]] |
| - | [[Category: Ion channel]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| Structural highlights
Function
A0A0R4I973_MNELE
Publication Abstract from PubMed
Recent genome projects for ctenophores have revealed the presence of numerous ionotropic glutamate receptors (iGluRs) in Mnemiopsis leidyi and Pleurobrachia bachei, among our earliest metazoan ancestors. Sequence alignments and phylogenetic analysis show that these form a distinct clade from the well-characterized AMPA, kainate, and NMDA iGluR subtypes found in vertebrates. Although annotated as glutamate and kainate receptors, crystal structures of the ML032222a and PbiGluR3 ligand-binding domains (LBDs) reveal endogenous glycine in the binding pocket, whereas ligand-binding assays show that glycine binds with nanomolar affinity; biochemical assays and structural analysis establish that glutamate is occluded from the binding cavity. Further analysis reveals ctenophore-specific features, such as an interdomain Arg-Glu salt bridge, present only in subunits that bind glycine, but also a conserved disulfide in loop 1 of the LBD that is found in all vertebrate NMDA but not AMPA or kainate receptors. We hypothesize that ctenophore iGluRs are related to an early ancestor of NMDA receptors, suggesting a common evolutionary path for ctenophores and bilaterian species, and suggest that future work should consider both glycine and glutamate as candidate neurotransmitters in ctenophore species.
Glycine activated ion channel subunits encoded by ctenophore glutamate receptor genes.,Alberstein R, Grey R, Zimmet A, Simmons DK, Mayer ML Proc Natl Acad Sci U S A. 2015 Nov 3;112(44):E6048-57. doi:, 10.1073/pnas.1513771112. Epub 2015 Oct 12. PMID:26460032[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Alberstein R, Grey R, Zimmet A, Simmons DK, Mayer ML. Glycine activated ion channel subunits encoded by ctenophore glutamate receptor genes. Proc Natl Acad Sci U S A. 2015 Nov 3;112(44):E6048-57. doi:, 10.1073/pnas.1513771112. Epub 2015 Oct 12. PMID:26460032 doi:http://dx.doi.org/10.1073/pnas.1513771112
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