1emt

From Proteopedia

(Difference between revisions)

Current revision

FAB ANTIBODY FRAGMENT OF AN C60 ANTIFULLERENE ANTIBODY

Structural highlights

1emt is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.25Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

IGKC_MOUSE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We have prepared a monoclonal Buckminsterfullerene specific antibody and report the sequences of its light and heavy chains. We also show, by x-ray crystallographic analysis of the Fab fragment and by model building, that the fullerene binding site is formed by the interface of the antibody light and heavy chains. Shape-complementary clustering of hydrophobic amino acids, several of which participate in putative stacking interactions with fullerene, form the binding site. Moreover, an induced fit mechanism appears to participate in the fullerene binding process. Affinity of the antibody-fullerene complex is 22 nM as measured by competitive binding. These findings should be applicable not only to the use of antibodies to assay and direct potential fullerene-based drug design but could also lead to new methodologies for the production of fullerene derivatives and nanotubes as well.

X-ray crystal structure of an anti-Buckminsterfullerene antibody fab fragment: biomolecular recognition of C(60).,Braden BC, Goldbaum FA, Chen BX, Kirschner AN, Wilson SR, Erlanger BF Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12193-7. PMID:11035793^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Braden BC, Goldbaum FA, Chen BX, Kirschner AN, Wilson SR, Erlanger BF. X-ray crystal structure of an anti-Buckminsterfullerene antibody fab fragment: biomolecular recognition of C(60). Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12193-7. PMID:11035793 doi:10.1073/pnas.210396197

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1emt"

@@ Line 3: / Line 3: @@
 <StructureSection load='1emt' size='340' side='right'caption='[[1emt]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1emt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EMT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EMT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1emt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EMT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EMT FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1emt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1emt OCA], [http://pdbe.org/1emt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1emt RCSB], [http://www.ebi.ac.uk/pdbsum/1emt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1emt ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1emt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1emt OCA], [https://pdbe.org/1emt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1emt RCSB], [https://www.ebi.ac.uk/pdbsum/1emt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1emt ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/IGKC_MOUSE IGKC_MOUSE]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 11: / Line 14: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/em/1emt_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 34: / Line 37: @@
 [[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Braden, B C]]
+[[Category: Braden BC]]
-[[Category: Chen, B X]]
+[[Category: Chen B-X]]
-[[Category: Erlanger, B F]]
+[[Category: Erlanger BF]]
-[[Category: Goldbaum, F A]]
+[[Category: Goldbaum FA]]
-[[Category: Kirschner, A N]]
+[[Category: Kirschner AN]]
-[[Category: Wilson, S R]]
+[[Category: Wilson SR]]
-[[Category: Anti-fullerene antibody]]
-[[Category: Immune system]]
-[[Category: Nanotube]]

1emt

From Proteopedia

Current revision

FAB ANTIBODY FRAGMENT OF AN C60 ANTIFULLERENE ANTIBODY

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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