DnaA
From Proteopedia
(Difference between revisions)
| (One intermediate revision not shown.) | |||
| Line 1: | Line 1: | ||
<StructureSection load='' size='350' side='right' scene='50/509255/Cv/1' caption='E. coli DnaA complex with DNA ([[1j1v]])'> | <StructureSection load='' size='350' side='right' scene='50/509255/Cv/1' caption='E. coli DnaA complex with DNA ([[1j1v]])'> | ||
| - | '''DnaA''' activates initiation of DNA replication in prokaryotes. DnaA promotes the unwinding of the DNA helix. The cellular concentration of DnaA determines the initiation of DNA replication. Active DnaA is bound to ATP. DnaA binds to DNA at a specific 9 base pair DnaA box in oriC (origin of replication sequence). <ref>PMID:17435790</ref> | + | |
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | '''DnaA''' or '''chromosomal replication initiator protein DnaA''' activates initiation of DNA replication in prokaryotes. DnaA promotes the unwinding of the DNA helix. The cellular concentration of DnaA determines the initiation of DNA replication. Active DnaA is bound to ATP. DnaA binds to DNA at a specific 9 base pair DnaA box in oriC (origin of replication sequence). <ref>PMID:17435790</ref> | ||
<scene name='50/509255/Cv/2'>Protein-DNA interface</scene> ([[1j1v]]).<ref>PMID:12682358</ref> | <scene name='50/509255/Cv/2'>Protein-DNA interface</scene> ([[1j1v]]).<ref>PMID:12682358</ref> | ||
| Line 19: | Line 39: | ||
[[2wp0]] - DnaA N terminal + HOBA – ''Helicobacter pylori''<br /> | [[2wp0]] - DnaA N terminal + HOBA – ''Helicobacter pylori''<br /> | ||
[[3pvp]], [[3pvv]] - DnaA DNA binding domain + DNA – ''Mycobacterium tuberculosis''<br /> | [[3pvp]], [[3pvv]] - DnaA DNA binding domain + DNA – ''Mycobacterium tuberculosis''<br /> | ||
| - | [[4tps]] - | + | [[4tps]] - BsDnaA domain I 1-82 + SirA – ''Bacillus subtilis''<br /> |
| + | [[8bv3]] - BsDnaA domain III 106-346<br /> | ||
| + | [[8btg]] - BsDnaA + DNA – Cryo EM<br /> | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
| |||||||||||
3D structures of DnaA
Updated on 27-April-2025
1l8q – AaDnaA + ADP – Aquifex aeolicus
2hcb - AaDnaA + AMPPCP
3r8f - AaDnaA DNA binding domain + DNA
1j1v – EcDnaA DNA binding domain + DNA – Escherichia coli
2e0g - EcDnaA N terminal – NMR
2jmp - DnaA N terminal – Mycoplasma genitalium – NMR
2z4r, 2z4s - DnaA domain III – Thermotoga maritima
2wp0 - DnaA N terminal + HOBA – Helicobacter pylori
3pvp, 3pvv - DnaA DNA binding domain + DNA – Mycobacterium tuberculosis
4tps - BsDnaA domain I 1-82 + SirA – Bacillus subtilis
8bv3 - BsDnaA domain III 106-346
8btg - BsDnaA + DNA – Cryo EM
References
- ↑ Mott ML, Berger JM. DNA replication initiation: mechanisms and regulation in bacteria. Nat Rev Microbiol. 2007 May;5(5):343-54. PMID:17435790 doi:http://dx.doi.org/10.1038/nrmicro1640
- ↑ Fujikawa N, Kurumizaka H, Nureki O, Terada T, Shirouzu M, Katayama T, Yokoyama S. Structural basis of replication origin recognition by the DnaA protein. Nucleic Acids Res. 2003 Apr 15;31(8):2077-86. PMID:12682358
