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| | ==Conformational switches control early maturation of the eukaryotic small ribosomal subunit== | | ==Conformational switches control early maturation of the eukaryotic small ribosomal subunit== |
| - | <StructureSection load='6nd4' size='340' side='right'caption='[[6nd4]], [[Resolution|resolution]] 4.30Å' scene=''> | + | <SX load='6nd4' size='340' side='right' viewer='molstar' caption='[[6nd4]], [[Resolution|resolution]] 4.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6nd4]] is a 29 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_by4741 Saccharomyces cerevisiae by4741]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ND4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ND4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6nd4]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_BY4741 Saccharomyces cerevisiae BY4741]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ND4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ND4 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=N:ANY+5-MONOPHOSPHATE+NUCLEOTIDE'>N</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.3Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nd4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nd4 OCA], [http://pdbe.org/6nd4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nd4 RCSB], [http://www.ebi.ac.uk/pdbsum/6nd4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nd4 ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=N:ANY+5-MONOPHOSPHATE+NUCLEOTIDE'>N</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nd4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nd4 OCA], [https://pdbe.org/6nd4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nd4 RCSB], [https://www.ebi.ac.uk/pdbsum/6nd4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nd4 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PWP2_YEAST PWP2_YEAST]] Required for bud-site selection and cell separation. Also involved in nucleolar processing of pre-18S ribosomal RNA.<ref>PMID:12068309</ref> <ref>PMID:8804409</ref> [[http://www.uniprot.org/uniprot/BUD21_YEAST BUD21_YEAST]] Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in bud site selection maybe via the regulation of expression of bipolar budding components.<ref>PMID:11452010</ref> <ref>PMID:12068309</ref> [[http://www.uniprot.org/uniprot/RRP9_YEAST RRP9_YEAST]] Involved in nucleolar processing of pre-18S ribosomal RNA. Required for efficient pre-rRNA cleavage at sites A0, A1 and A2, and biosynthesis of 18S rRNA.<ref>PMID:11105764</ref> [[http://www.uniprot.org/uniprot/DCA13_YEAST DCA13_YEAST]] Required for ribosomal RNA processing.<ref>PMID:8508778</ref> [[http://www.uniprot.org/uniprot/UTP7_YEAST UTP7_YEAST]] Involved in nucleolar processing of pre-18S ribosomal RNA.<ref>PMID:12068309</ref> [[http://www.uniprot.org/uniprot/UTP21_YEAST UTP21_YEAST]] Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.<ref>PMID:15590835</ref> [[http://www.uniprot.org/uniprot/FCF1_YEAST FCF1_YEAST]] Essential protein involved in pre-rRNA processing and 40S ribosomal subunit assembly. Required for the early cleavage steps of 35S rRNA at the A(0), A(1), and A(2) sites.<ref>PMID:16762320</ref> [[http://www.uniprot.org/uniprot/SNU13_YEAST SNU13_YEAST]] Common component of the spliceosome and rRNA processing machinery. In association with the spliceosomal U4/U6.U5 tri-snRNP particle, required for splicing of pre-mRNA. In association with box C/D snoRNPs, required for processing of pre-ribosomal RNA (rRNA) and site-specific 2'-O-methylation of substrate RNAs. Essential for the accumulation and stability of U4 snRNA, U6 snRNA, and box C/D snoRNAs.<ref>PMID:11081632</ref> <ref>PMID:12215523</ref> <ref>PMID:14730029</ref> [[http://www.uniprot.org/uniprot/UTP15_YEAST UTP15_YEAST]] Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs).<ref>PMID:12068309</ref> <ref>PMID:15489292</ref> [[http://www.uniprot.org/uniprot/UTP10_YEAST UTP10_YEAST]] Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs). Involved in ribosome biosynthesis.<ref>PMID:12068309</ref> <ref>PMID:15489292</ref> <ref>PMID:16544271</ref> <ref>PMID:17652137</ref> [[http://www.uniprot.org/uniprot/MPP10_YEAST MPP10_YEAST]] Involved in nucleolar processing of pre-18S ribosomal RNA. Required for the early cleavages at sites A0, A1 and A2 during 18S ribosomal pre-RNA processing.<ref>PMID:15489263</ref> <ref>PMID:9315638</ref> [[http://www.uniprot.org/uniprot/UTP18_YEAST UTP18_YEAST]] Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.<ref>PMID:15590835</ref> [[http://www.uniprot.org/uniprot/UTP5_YEAST UTP5_YEAST]] Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs).<ref>PMID:12068309</ref> <ref>PMID:15489292</ref> [[http://www.uniprot.org/uniprot/FBRL_YEAST FBRL_YEAST]] S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed:1825809). Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Involved in the biogenesis of the 18S rRNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).<ref>PMID:1825809</ref> <ref>PMID:24352239</ref> <ref>PMID:2686980</ref> [[http://www.uniprot.org/uniprot/IMP3_YEAST IMP3_YEAST]] Required for the early cleavages at sites A0, A1 and A2 during 18S ribosomal pre-RNA processing.<ref>PMID:10409734</ref> <ref>PMID:15489263</ref> | + | [https://www.uniprot.org/uniprot/UTP17_YEAST UTP17_YEAST] Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs).<ref>PMID:12068309</ref> <ref>PMID:15489292</ref> |
| | + | |
| | + | ==See Also== |
| | + | *[[Ribosome 3D structures|Ribosome 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Saccharomyces cerevisiae by4741]] | + | [[Category: Saccharomyces cerevisiae BY4741]] |
| - | [[Category: Barandun, J]] | + | [[Category: Barandun J]] |
| - | [[Category: Hunziker, M]] | + | [[Category: Hunziker M]] |
| - | [[Category: Klinge, S]] | + | [[Category: Klinge S]] |
| - | [[Category: Ribosome]]
| + | |
| - | [[Category: Ribosome assembly]]
| + | |
| Structural highlights
Function
UTP17_YEAST Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs).[1] [2]
See Also
References
- ↑ Dragon F, Gallagher JE, Compagnone-Post PA, Mitchell BM, Porwancher KA, Wehner KA, Wormsley S, Settlage RE, Shabanowitz J, Osheim Y, Beyer AL, Hunt DF, Baserga SJ. A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis. Nature. 2002 Jun 27;417(6892):967-70. Epub 2002 Jun 9. PMID:12068309 doi:http://dx.doi.org/10.1038/nature00769
- ↑ Gallagher JE, Dunbar DA, Granneman S, Mitchell BM, Osheim Y, Beyer AL, Baserga SJ. RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components. Genes Dev. 2004 Oct 15;18(20):2506-17. PMID:15489292 doi:http://dx.doi.org/18/20/2506
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