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6ro5
From Proteopedia
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<StructureSection load='6ro5' size='340' side='right'caption='[[6ro5]], [[Resolution|resolution]] 1.68Å' scene=''> | <StructureSection load='6ro5' size='340' side='right'caption='[[6ro5]], [[Resolution|resolution]] 1.68Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6ro5]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[6ro5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RO5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RO5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=QEB:[Re4(mu3-OH)4(CO)12]'>QEB</scene>, <scene name='pdbligand=RE:RHENIUM'>RE</scene>, <scene name='pdbligand=RRE:fac-tricarbonyl-triaqua+rhenium(I)'>RRE</scene | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.68Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=QEB:[Re4(mu3-OH)4(CO)12]'>QEB</scene>, <scene name='pdbligand=RE:RHENIUM'>RE</scene>, <scene name='pdbligand=RRE:fac-tricarbonyl-triaqua+rhenium(I)'>RRE</scene></td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ro5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ro5 OCA], [https://pdbe.org/6ro5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ro5 RCSB], [https://www.ebi.ac.uk/pdbsum/6ro5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ro5 ProSAT]</span></td></tr> | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The fact that a protein crystal can serve as a chemical reaction vessel is intrinsically fascinating. That it can produce an electron-dense tetranuclear rhenium cluster compound from a rhenium tri-carbonyl tri-bromo starting compound adds to the fascination. Such a cluster has been synthesized previously in vitro, where it formed under basic conditions. Therefore, its synthesis in a protein crystal grown at pH 4.5 is even more unexpected. The X-ray crystal structures presented here are for the protein hen egg-white lysozyme incubated with a rhenium tri-carbonyl tri-bromo compound for periods of one and two years. These reveal a completed, very well resolved, tetra-rhenium cluster after two years and an intermediate state, where the carbonyl ligands to the rhenium cluster are not yet clearly resolved, after one year. A dense tetranuclear rhenium cluster, and its technetium form, offer enhanced contrast in medical imaging. Stimulated by these crystallography results, the unusual formation of such a species directly in an in vivo situation has been considered. It offers a new option for medical imaging compounds, particularly when considering the application of the pre-formed tetranuclear cluster, suggesting that it may be suitable for medical diagnosis because of its stability, preference of formation and biological compatibility. | ||
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| + | Formation of a highly dense tetra-rhenium cluster in a protein crystal and its implications in medical imaging.,Brink A, Helliwell JR IUCrJ. 2019 Jun 13;6(Pt 4):695-702. doi: 10.1107/S2052252519006651. eCollection, 2019 Jul 1. PMID:31316813<ref>PMID:31316813</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6ro5" style="background-color:#fffaf0;"></div> | ||
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| + | ==See Also== | ||
| + | *[[Lysozyme 3D structures|Lysozyme 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | + | [[Category: Brink A]] | |
| - | [[Category: Brink | + | [[Category: Helliwell JR]] |
| - | [[Category: Helliwell | + | |
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Current revision
1Yr-Y: Lysozyme with Re Cluster 1 year on shelf
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