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| ==Structure of connexin-50 intercellular gap junction channel at 3.4 angstrom resolution by cryoEM== | | ==Structure of connexin-50 intercellular gap junction channel at 3.4 angstrom resolution by cryoEM== |
- | <StructureSection load='6mhy' size='340' side='right'caption='[[6mhy]], [[Resolution|resolution]] 3.40Å' scene=''> | + | <SX load='6mhy' size='340' side='right' viewer='molstar' caption='[[6mhy]], [[Resolution|resolution]] 3.40Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
- | <table><tr><td colspan='2'>[[6mhy]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MHY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6mhy]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Ovis_aries Ovis aries]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MHY FirstGlance]. <br> |
- | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mhy OCA], [http://pdbe.org/6mhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mhy RCSB], [http://www.ebi.ac.uk/pdbsum/6mhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mhy ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mhy OCA], [https://pdbe.org/6mhy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mhy RCSB], [https://www.ebi.ac.uk/pdbsum/6mhy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mhy ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function == | | == Function == |
- | [[http://www.uniprot.org/uniprot/CXA8_SHEEP CXA8_SHEEP]] One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. | + | [https://www.uniprot.org/uniprot/CXA8_SHEEP CXA8_SHEEP] One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. |
| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| <references/> | | <references/> |
| __TOC__ | | __TOC__ |
- | </StructureSection> | + | </SX> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Ovis aries]] | | [[Category: Ovis aries]] |
- | [[Category: Myers, J B]] | + | [[Category: Myers JB]] |
- | [[Category: Reichow, S L]] | + | [[Category: Reichow SL]] |
- | [[Category: Cell communication]]
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- | [[Category: Connexin]]
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- | [[Category: Gap junction]]
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- | [[Category: Ion channel]]
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- | [[Category: Membrane protein]]
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| Structural highlights
Function
CXA8_SHEEP One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Publication Abstract from PubMed
Gap junctions establish direct pathways for cell-to-cell communication through the assembly of twelve connexin subunits that form intercellular channels connecting neighbouring cells. Co-assembly of different connexin isoforms produces channels with unique properties and enables communication across cell types. Here we used single-particle cryo-electron microscopy to investigate the structural basis of connexin co-assembly in native lens gap junction channels composed of connexin 46 and connexin 50 (Cx46/50). We provide the first comparative analysis to connexin 26 (Cx26), which-together with computational studies-elucidates key energetic features governing gap junction permselectivity. Cx46/50 adopts an open-state conformation that is distinct from the Cx26 crystal structure, yet it appears to be stabilized by a conserved set of hydrophobic anchoring residues. 'Hot spots' of genetic mutations linked to hereditary cataract formation map to the core structural-functional elements identified in Cx46/50, suggesting explanations for many of the disease-causing effects.
Structure of native lens connexin 46/50 intercellular channels by cryo-EM.,Myers JB, Haddad BG, O'Neill SE, Chorev DS, Yoshioka CC, Robinson CV, Zuckerman DM, Reichow SL Nature. 2018 Dec;564(7736):372-377. doi: 10.1038/s41586-018-0786-7. Epub 2018 Dec, 12. PMID:30542154[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Myers JB, Haddad BG, O'Neill SE, Chorev DS, Yoshioka CC, Robinson CV, Zuckerman DM, Reichow SL. Structure of native lens connexin 46/50 intercellular channels by cryo-EM. Nature. 2018 Dec;564(7736):372-377. doi: 10.1038/s41586-018-0786-7. Epub 2018 Dec, 12. PMID:30542154 doi:http://dx.doi.org/10.1038/s41586-018-0786-7
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