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| | <StructureSection load='4z7f' size='340' side='right'caption='[[4z7f]], [[Resolution|resolution]] 3.19Å' scene=''> | | <StructureSection load='4z7f' size='340' side='right'caption='[[4z7f]], [[Resolution|resolution]] 3.19Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4z7f]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Entfa Entfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z7F FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4z7f]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis_V583 Enterococcus faecalis V583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z7F FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FOL:FOLIC+ACID'>FOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.194Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EF_0940 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=226185 ENTFA])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FOL:FOLIC+ACID'>FOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z7f OCA], [http://pdbe.org/4z7f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z7f RCSB], [http://www.ebi.ac.uk/pdbsum/4z7f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z7f ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z7f OCA], [https://pdbe.org/4z7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z7f RCSB], [https://www.ebi.ac.uk/pdbsum/4z7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z7f ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q837A3_ENTFA Q837A3_ENTFA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Entfa]] | + | [[Category: Enterococcus faecalis V583]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Wang, C C]] | + | [[Category: Wang CC]] |
| - | [[Category: Wang, C Y]] | + | [[Category: Wang CY]] |
| - | [[Category: Zhang, P]] | + | [[Category: Zhang P]] |
| - | [[Category: Zhao, Q]] | + | [[Category: Zhao Q]] |
| - | [[Category: Atp-binding cassette transporter]]
| + | |
| - | [[Category: Folate binding and release]]
| + | |
| - | [[Category: Folate transporter]]
| + | |
| - | [[Category: Gating mechanism]]
| + | |
| - | [[Category: Group ii ecf transporter]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
Q837A3_ENTFA
Publication Abstract from PubMed
Energy-coupling factor (ECF) transporters are a new family of ABC transporters that consist of four subunits, two cytoplasmic ATPases EcfA and EcfA' and two transmembrane proteins namely EcfS for substrate-specific binding and EcfT for energy coupling. Here, we report the 3.2-A resolution crystal structure of the EcfS protein of a folate ECF transporter from Enterococcus faecalis-EfFolT, a close homologue of FolT from Lactobacillus brevis-LbFolT. Structural and biochemical analyses reveal the residues constituting the folate-binding pocket and determining the substrate-binding specificity. Structural comparison of the folate-bound EfFolT with the folate-free LbFolT contained in the holotransporter complex discloses significant conformational change at the L1 loop, and reveals a gating mechanism of ECF transporters in which the L1 loop of EcfS acts as a gate in the substrate binding and release.
Structures of FolT in substrate-bound and substrate-released conformations reveal a gating mechanism for ECF transporters.,Zhao Q, Wang C, Wang C, Guo H, Bao Z, Zhang M, Zhang P Nat Commun. 2015 Jul 22;6:7661. doi: 10.1038/ncomms8661. PMID:26198469[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhao Q, Wang C, Wang C, Guo H, Bao Z, Zhang M, Zhang P. Structures of FolT in substrate-bound and substrate-released conformations reveal a gating mechanism for ECF transporters. Nat Commun. 2015 Jul 22;6:7661. doi: 10.1038/ncomms8661. PMID:26198469 doi:http://dx.doi.org/10.1038/ncomms8661
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