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| | <StructureSection load='4ywv' size='340' side='right'caption='[[4ywv]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='4ywv' size='340' side='right'caption='[[4ywv]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ywv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_bei_nr-33708 Streptococcus pyogenes bei nr-33708]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YWV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YWV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ywv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_MGAS1882 Streptococcus pyogenes MGAS1882]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YWV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YWV FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SSN:4-OXOBUTANOIC+ACID'>SSN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ogd|4ogd]], [[4oht|4oht]], [[4ywu|4ywu]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SSN:4-OXOBUTANOIC+ACID'>SSN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gabD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1010840 Streptococcus pyogenes BEI NR-33708])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ywv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ywv OCA], [https://pdbe.org/4ywv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ywv RCSB], [https://www.ebi.ac.uk/pdbsum/4ywv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ywv ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Succinate-semialdehyde_dehydrogenase_(NADP(+)) Succinate-semialdehyde dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.79 1.2.1.79] </span></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ywv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ywv OCA], [http://pdbe.org/4ywv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ywv RCSB], [http://www.ebi.ac.uk/pdbsum/4ywv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ywv ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0J9X1M8_STRPY A0A0J9X1M8_STRPY] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Streptococcus pyogenes bei nr-33708]] | + | [[Category: Streptococcus pyogenes MGAS1882]] |
| - | [[Category: Chi, Y M]] | + | [[Category: Chi YM]] |
| - | [[Category: Jang, E H]] | + | [[Category: Jang EH]] |
| - | [[Category: Lee, K S]] | + | [[Category: Lee KS]] |
| - | [[Category: Park, S A]] | + | [[Category: Park SA]] |
| - | [[Category: Ess-complex structure]]
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| - | [[Category: Oxidoreductase]]
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| - | [[Category: Streptococcus pyogene]]
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| - | [[Category: Substrate inhibition]]
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| - | [[Category: Succinic semialdehyde dehydrogenase]]
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| Structural highlights
Function
A0A0J9X1M8_STRPY
Publication Abstract from PubMed
Succinic semialdehyde dehydrogenases (SSADHs) are ubiquitous enzymes that catalyze the oxidation of succinic semialdehyde (SSA) to succinic acid in the presence of NAD(P)+, and play an important role in the cellular mechanisms including the detoxification of accumulated SSA or the survival in conditions of limited nutrients. Here, we report the inhibitory properties and two crystal structures of SSADH from Streptococcus pyogenes (SpSSADH) in a binary (ES) complex with SSA as the substrate and a ternary (ESS) complex with the substrate SSA and the inhibitory SSA, at 2.4 A resolution for both structures. Analysis of the kinetic inhibitory parameters revealed significant substrate inhibition in the presence of NADP+ at concentrations of SSA higher than 0.02 mM, which exhibited complete uncompetitive substrate inhibition with the inhibition constant (Ki) value of 0.10 +/- 0.02 mM. In ES-complex of SpSSADH, the SSA showed a tightly bound bent form nearby the catalytic residues, which may be caused by reduction of the cavity volume for substrate binding, compared with other SSADHs. Moreover, structural comparison of ESS-complex with a binary complex with NADP+ of SpSSADH indicated that the substrate inhibition was induced by the binding of inhibitory SSA in the cofactor-binding site, instead of NADP+. Our results provide first structure-based molecular insights into the substrate inhibition mechanism of SpSSADH as the Gram-positive bacterial SSADH.
Structural insight into the substrate inhibition mechanism of NADP-dependent succinic semialdehyde dehydrogenase from Streptococcus pyogenes.,Jang EH, Park SA, Chi YM, Lee KS Biochem Biophys Res Commun. 2015 Apr 16. pii: S0006-291X(15)00725-1. doi:, 10.1016/j.bbrc.2015.04.047. PMID:25888791[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Jang EH, Park SA, Chi YM, Lee KS. Structural insight into the substrate inhibition mechanism of NADP-dependent succinic semialdehyde dehydrogenase from Streptococcus pyogenes. Biochem Biophys Res Commun. 2015 Apr 16. pii: S0006-291X(15)00725-1. doi:, 10.1016/j.bbrc.2015.04.047. PMID:25888791 doi:http://dx.doi.org/10.1016/j.bbrc.2015.04.047
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