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| | <StructureSection load='4z9e' size='340' side='right'caption='[[4z9e]], [[Resolution|resolution]] 2.49Å' scene=''> | | <StructureSection load='4z9e' size='340' side='right'caption='[[4z9e]], [[Resolution|resolution]] 2.49Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4z9e]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thevo Thevo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z9E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z9E FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4z9e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoplasma_volcanium_GSS1 Thermoplasma volcanium GSS1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z9E FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">albA, TV1085, TVG1115434 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273116 THEVO])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.49Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z9e OCA], [http://pdbe.org/4z9e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z9e RCSB], [http://www.ebi.ac.uk/pdbsum/4z9e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z9e ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z9e OCA], [https://pdbe.org/4z9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z9e RCSB], [https://www.ebi.ac.uk/pdbsum/4z9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z9e ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ALBA_THEVO ALBA_THEVO]] Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes. | + | [https://www.uniprot.org/uniprot/ALBA_THEVO ALBA_THEVO] Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Thevo]] | + | [[Category: Thermoplasma volcanium GSS1]] |
| - | [[Category: Lee, B J]] | + | [[Category: Lee BJ]] |
| - | [[Category: Lee, S J]] | + | [[Category: Lee SJ]] |
| - | [[Category: Ma, C]] | + | [[Category: Ma C]] |
| - | [[Category: Pathak, C]] | + | [[Category: Pathak C]] |
| - | [[Category: Acetyltransferase]]
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| - | [[Category: Dna binding protein]]
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| - | [[Category: Thermoplasma volcanium]]
| + | |
| Structural highlights
Function
ALBA_THEVO Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes.
Publication Abstract from PubMed
The Alba superfamily proteins have been regarded as a conserved group of proteins in archaea and eukarya, which have shown to be important in nucleic acid binding, chromatic organization and gene regulation. These proteins often belong to the N-acetyltransferase (NAT) category (N(alpha)-acetyltransferases or N(epsilon)-acetyltransferases) and undergo post-translational modifications. Here, we report the crystal structure of Alba from Thermoplasma volcanium (Tv Alba) at 2.4 A resolution. The acetylation of Tv Alba was monitored and the N-terminal of Tv Alba has been shown to interact with acetyl coenzyme A (Ac-CoA). The chemical shift perturbation experiments of Tv Alba were performed in the presence of Ac-CoA and/or Tv Ard1, another T. volcanium protein that treats Tv Alba as a substrate. To examine the DNA binding capabilities of Tv Alba alone and in the presence of Ac-CoA and/or Tv Ard1, EMSA experiments were carried out. It is shown that although Tv Alba binds to Ac-CoA, the acetylation of Tv Alba is not related with its binding to dsDNA, and the involvement of the N-terminus in Ac-CoA binding demonstrates that Tv Alba belongs to the N(alpha)-acetyltransferase family.
Alba from Thermoplasma volcanium belongs to alpha-NAT's: An insight into the structural aspects of Tv Alba and its acetylation by Tv Ard1.,Ma C, Pathak C, Lee SJ, Lee KY, Jang SB, Nam M, Im H, Yoon HJ, Lee BJ Arch Biochem Biophys. 2016 Jan 15;590:90-100. doi: 10.1016/j.abb.2015.11.039., Epub 2015 Nov 30. PMID:26657068[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ma C, Pathak C, Lee SJ, Lee KY, Jang SB, Nam M, Im H, Yoon HJ, Lee BJ. Alba from Thermoplasma volcanium belongs to alpha-NAT's: An insight into the structural aspects of Tv Alba and its acetylation by Tv Ard1. Arch Biochem Biophys. 2016 Jan 15;590:90-100. doi: 10.1016/j.abb.2015.11.039., Epub 2015 Nov 30. PMID:26657068 doi:http://dx.doi.org/10.1016/j.abb.2015.11.039
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