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| | <StructureSection load='4zi5' size='340' side='right'caption='[[4zi5]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='4zi5' size='340' side='right'caption='[[4zi5]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4zi5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Environmental_sequence Environmental sequence]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZI5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZI5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4zi5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Metagenome Metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZI5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.702Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryldialkylphosphatase Aryldialkylphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.1 3.1.8.1] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zi5 OCA], [http://pdbe.org/4zi5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zi5 RCSB], [http://www.ebi.ac.uk/pdbsum/4zi5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zi5 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zi5 OCA], [https://pdbe.org/4zi5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zi5 RCSB], [https://www.ebi.ac.uk/pdbsum/4zi5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zi5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aryldialkylphosphatase]] | |
| - | [[Category: Environmental sequence]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Colin, P Y]] | + | [[Category: Metagenome]] |
| - | [[Category: Fischer, G]] | + | [[Category: Colin P-Y]] |
| - | [[Category: Hollfelder, F]] | + | [[Category: Fischer G]] |
| - | [[Category: Hyvonen, M]] | + | [[Category: Hollfelder F]] |
| - | [[Category: Alpha/beta hydrolase]]
| + | [[Category: Hyvonen M]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Metagenomic library]]
| + | |
| - | [[Category: Phosphotriesterase]]
| + | |
| - | [[Category: Promiscuity]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Unculturable bacterial communities provide a rich source of biocatalysts, but their experimental discovery by functional metagenomics is difficult, because the odds are stacked against the experimentor. Here we demonstrate functional screening of a million-membered metagenomic library in microfluidic picolitre droplet compartments. Using bait substrates, new hydrolases for sulfate monoesters and phosphotriesters were identified, mostly based on promiscuous activities presumed not to be under selection pressure. Spanning three protein superfamilies, these break new ground in sequence space: promiscuity now connects enzymes with only distantly related sequences. Most hits could not have been predicted by sequence analysis, because the desired activities have never been ascribed to similar sequences, showing how this approach complements bioinformatic harvesting of metagenomic sequencing data. Functional screening of a library of unprecedented size with excellent assay sensitivity has been instrumental in identifying rare genes constituting catalytically versatile hubs in sequence space as potential starting points for the acquisition of new functions.
Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics.,Colin PY, Kintses B, Gielen F, Miton CM, Fischer G, Mohamed MF, Hyvonen M, Morgavi DP, Janssen DB, Hollfelder F Nat Commun. 2015 Dec 7;6:10008. doi: 10.1038/ncomms10008. PMID:26639611[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Colin PY, Kintses B, Gielen F, Miton CM, Fischer G, Mohamed MF, Hyvonen M, Morgavi DP, Janssen DB, Hollfelder F. Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics. Nat Commun. 2015 Dec 7;6:10008. doi: 10.1038/ncomms10008. PMID:26639611 doi:http://dx.doi.org/10.1038/ncomms10008
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