|
|
| (One intermediate revision not shown.) |
| Line 3: |
Line 3: |
| | <StructureSection load='4yvu' size='340' side='right'caption='[[4yvu]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='4yvu' size='340' side='right'caption='[[4yvu]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4yvu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YVU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YVU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4yvu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YVU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4yvn|4yvn]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cotA, pig, BSU06300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yvu OCA], [https://pdbe.org/4yvu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yvu RCSB], [https://www.ebi.ac.uk/pdbsum/4yvu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yvu ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yvu OCA], [http://pdbe.org/4yvu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yvu RCSB], [http://www.ebi.ac.uk/pdbsum/4yvu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4yvu ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/COTA_BACSU COTA_BACSU]] Involved in brown pigmentation during sporogenesis. | + | [https://www.uniprot.org/uniprot/COTA_BACSU COTA_BACSU] Involved in brown pigmentation during sporogenesis. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 20: |
Line 19: |
| | </div> | | </div> |
| | <div class="pdbe-citations 4yvu" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4yvu" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Laccase 3D structures|Laccase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacsu]] | + | [[Category: Bacillus subtilis subsp. subtilis str. 168]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Liu, Z C]] | + | [[Category: Liu ZC]] |
| - | [[Category: Wang, G G]] | + | [[Category: Wang GG]] |
| - | [[Category: Xie, T]] | + | [[Category: Xie T]] |
| - | [[Category: Laccase]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Spore coat protein some]]
| + | |
| Structural highlights
Function
COTA_BACSU Involved in brown pigmentation during sporogenesis.
Publication Abstract from PubMed
The CotA laccase from Bacillus subtilis is an abundant component of the spore outer coat and has been characterized as a typical laccase. The crystal structure of CotA complexed with 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) in a hole motif has been solved. The novel binding site was about 26 A away from the T1 binding pocket. Comparison with known structures of other laccases revealed that the hole is a specific feature of CotA. The key residues Arg476 and Ser360 were directly bound to ABTS. Site-directed mutagenesis studies revealed that the residues Arg146, Arg429 and Arg476, which are located at the bottom of the novel binding site, are essential for the oxidation of ABTS and syringaldazine. Specially, a Thr480Phe variant was identified to be almost 3.5 times more specific for ABTS than for syringaldazine compared with the wild type. These results suggest this novel binding site for ABTS could be a potential target for protein engineering of CotA laccases.
Crystal structure of CotA laccase complexed with 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) at a novel binding site.,Liu Z, Xie T, Zhong Q, Wang G Acta Crystallogr F Struct Biol Commun. 2016 Apr 1;72(Pt 4):328-35. doi:, 10.1107/S2053230X1600426X. Epub 2016 Mar 24. PMID:27050268[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Liu Z, Xie T, Zhong Q, Wang G. Crystal structure of CotA laccase complexed with 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) at a novel binding site. Acta Crystallogr F Struct Biol Commun. 2016 Apr 1;72(Pt 4):328-35. doi:, 10.1107/S2053230X1600426X. Epub 2016 Mar 24. PMID:27050268 doi:http://dx.doi.org/10.1107/S2053230X1600426X
|
