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| | ==SOLUTION STRUCTURE OF THE INSECTICIDAL CALCIUM CHANNEL BLOCKER OMEGA-ATRACOTOXIN-HV2A== | | ==SOLUTION STRUCTURE OF THE INSECTICIDAL CALCIUM CHANNEL BLOCKER OMEGA-ATRACOTOXIN-HV2A== |
| - | <StructureSection load='1g9p' size='340' side='right'caption='[[1g9p]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1g9p' size='340' side='right'caption='[[1g9p]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1g9p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hadronyche_versuta Hadronyche versuta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G9P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G9P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1g9p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hadronyche_versuta Hadronyche versuta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G9P FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g9p OCA], [http://pdbe.org/1g9p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1g9p RCSB], [http://www.ebi.ac.uk/pdbsum/1g9p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1g9p ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g9p OCA], [https://pdbe.org/1g9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g9p RCSB], [https://www.ebi.ac.uk/pdbsum/1g9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g9p ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TOT2A_HADVE TOT2A_HADVE]] Potent inhibitor of insect (bee brain), but not mammalian (rat trigeminal neurons), voltage-gated calcium channels (Cav). As for omega-AcTx-Hv1a, the phenotypic effect of injection of this toxin into lone star ticks (Amblyomma americanum) is curling of all eight legs into closed loops, followed by death.<ref>PMID:16330063</ref> | + | [https://www.uniprot.org/uniprot/TOT2A_HADVE TOT2A_HADVE] Potent inhibitor of insect (bee brain), but not mammalian (rat trigeminal neurons), voltage-gated calcium channels (Cav). As for omega-AcTx-Hv1a, the phenotypic effect of injection of this toxin into lone star ticks (Amblyomma americanum) is curling of all eight legs into closed loops, followed by death.<ref>PMID:16330063</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Hadronyche versuta]] | | [[Category: Hadronyche versuta]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: King, G F]] | + | [[Category: King GF]] |
| - | [[Category: Wang, X H]] | + | [[Category: Wang X-H]] |
| - | [[Category: Cystine knot]]
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| - | [[Category: Toxin]]
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| Structural highlights
Function
TOT2A_HADVE Potent inhibitor of insect (bee brain), but not mammalian (rat trigeminal neurons), voltage-gated calcium channels (Cav). As for omega-AcTx-Hv1a, the phenotypic effect of injection of this toxin into lone star ticks (Amblyomma americanum) is curling of all eight legs into closed loops, followed by death.[1]
Publication Abstract from PubMed
We have isolated a novel family of insect-selective neurotoxins that appear to be the most potent blockers of insect voltage-gated calcium channels reported to date. These toxins display exceptional phylogenetic specificity, with at least a 10,000-fold preference for insect versus vertebrate calcium channels. The structure of one of the toxins reveals a highly structured, disulfide-rich core and a structurally disordered C-terminal extension that is essential for channel blocking activity. Weak structural/functional homology with omega-agatoxin-IVA/B, the prototypic inhibitor of vertebrate P-type calcium channels, suggests that these two toxin families might share a similar mechanism of action despite their vastly different phylogenetic specificities.
Discovery and structure of a potent and highly specific blocker of insect calcium channels.,Wang XH, Connor M, Wilson D, Wilson HI, Nicholson GM, Smith R, Shaw D, Mackay JP, Alewood PF, Christie MJ, King GF J Biol Chem. 2001 Oct 26;276(43):40306-12. Epub 2001 Aug 24. PMID:11522785[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Mukherjee AK, Sollod BL, Wikel SK, King GF. Orally active acaricidal peptide toxins from spider venom. Toxicon. 2006 Feb;47(2):182-7. Epub 2005 Dec 5. PMID:16330063 doi:http://dx.doi.org/10.1016/j.toxicon.2005.10.011
- ↑ Wang XH, Connor M, Wilson D, Wilson HI, Nicholson GM, Smith R, Shaw D, Mackay JP, Alewood PF, Christie MJ, King GF. Discovery and structure of a potent and highly specific blocker of insect calcium channels. J Biol Chem. 2001 Oct 26;276(43):40306-12. Epub 2001 Aug 24. PMID:11522785 doi:http://dx.doi.org/10.1074/jbc.M105206200
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