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| | <StructureSection load='1f94' size='340' side='right'caption='[[1f94]], [[Resolution|resolution]] 0.97Å' scene=''> | | <StructureSection load='1f94' size='340' side='right'caption='[[1f94]], [[Resolution|resolution]] 0.97Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1f94]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_candidus Bungarus candidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F94 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F94 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1f94]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bungarus_candidus Bungarus candidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F94 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F94 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f94 OCA], [http://pdbe.org/1f94 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1f94 RCSB], [http://www.ebi.ac.uk/pdbsum/1f94 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1f94 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.97Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f94 OCA], [https://pdbe.org/1f94 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f94 RCSB], [https://www.ebi.ac.uk/pdbsum/1f94 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f94 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BUCA_BUNCA BUCA_BUNCA]] Acts presynaptically. | + | [https://www.uniprot.org/uniprot/3NOJ_BUNCA 3NOJ_BUNCA] This toxin is described as enhancing presynaptic acetylcholine release, but neither experimental results, nor references to other sources are available.<ref>PMID:11053837</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Toxin Tx7335|Toxin Tx7335]] | + | *[[Bucandin|Bucandin]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | [[Category: Bungarus candidus]] | | [[Category: Bungarus candidus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Comoso, S]] | + | [[Category: Comoso S]] |
| - | [[Category: Deacon, A M]] | + | [[Category: Deacon AM]] |
| - | [[Category: Kini, R M]] | + | [[Category: Kini RM]] |
| - | [[Category: Kolatkar, P R]] | + | [[Category: Kolatkar PR]] |
| - | [[Category: Kuhn, P]] | + | [[Category: Kuhn P]] |
| - | [[Category: Rajaseger, G]] | + | [[Category: Rajaseger G]] |
| - | [[Category: Uson, I]] | + | [[Category: Uson I]] |
| - | [[Category: Three-finger snake presynaptic neurotoxin]]
| + | |
| - | [[Category: Toxin]]
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| Structural highlights
Function
3NOJ_BUNCA This toxin is described as enhancing presynaptic acetylcholine release, but neither experimental results, nor references to other sources are available.[1]
Publication Abstract from PubMed
Bucandin is a novel presynaptic neurotoxin isolated from Bungarus candidus (Malayan krait). It has the unique property of enhancing presynaptic acetylcholine release and represents a family of three-finger toxins with an additional disulfide in the first loop. There are no existing structures from this sub-category of three-finger toxins. The X-ray crystal structure of bucandin has been determined by the Shake-and-Bake direct-methods procedure. The resulting electron-density maps were of outstanding quality and allowed the automated tracing of 61 of the 63 amino-acid residues, including their side chains, and the placement of 48 solvent molecules. The 0.97 A resolution full-matrix least-squares refinement converged to a crystallographic R factor of 12.4% and the final model contains 118 solvent molecules. This is the highest resolution structure of any member of the three-finger toxin family and thus it can serve as the best model for other members of the family. Furthermore, the structure of this novel toxin will help in understanding its unique ability to enhance acetylcholine release. The unique structure resulting from the fifth disulfide bond residing in the first loop improves the understanding of other toxins with a similar arrangement of disulfide bonds.
The atomic resolution structure of bucandin, a novel toxin isolated from the Malayan krait, determined by direct methods.,Kuhn P, Deacon AM, Comoso S, Rajaseger G, Kini RM, Uson I, Kolatkar PR Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1401-7. PMID:11053837[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kuhn P, Deacon AM, Comoso S, Rajaseger G, Kini RM, Uson I, Kolatkar PR. The atomic resolution structure of bucandin, a novel toxin isolated from the Malayan krait, determined by direct methods. Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1401-7. PMID:11053837
- ↑ Kuhn P, Deacon AM, Comoso S, Rajaseger G, Kini RM, Uson I, Kolatkar PR. The atomic resolution structure of bucandin, a novel toxin isolated from the Malayan krait, determined by direct methods. Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1401-7. PMID:11053837
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