Exoenzyme
From Proteopedia
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<StructureSection load='2c8c' size='400' side='right' caption='Structure of exoenzyme C3 catalytic domain complex with ADP and NAD (PDB code [[2c8c]]).' scene='59/593306/Cv/8'> | <StructureSection load='2c8c' size='400' side='right' caption='Structure of exoenzyme C3 catalytic domain complex with ADP and NAD (PDB code [[2c8c]]).' scene='59/593306/Cv/8'> | ||
| - | '''Exoenzymes''' (Exo) are secreted by cells and break large macromolecules outside the cells. The breakdown components can pass through the cell wall. '''Exo C3''' is a ''Clostridium butulinum'' toxin which binds [[NAD]], splits it into ADP-ribose and nicotinamide and transfers the ADP-ribose to a protein substrate. | + | '''Exoenzymes''' (Exo) are secreted by cells and break large macromolecules outside the cells. The breakdown components can pass through the cell wall. |
| + | *'''Exo C3''' is a ''Clostridium butulinum'' toxin which binds [[NAD]], splits it into ADP-ribose and nicotinamide and transfers the ADP-ribose to a protein substrate. | ||
| + | * '''Exo S''' and '''Exo T''' are secreted by ''Pseudomonas aeruginosa'' and has GTPase-activating activity as well as ADP-ribosyltransferase activity<ref>PMID:11114250</ref> . | ||
<scene name='59/593306/Cv/6'>NAD binding site</scene>. | <scene name='59/593306/Cv/6'>NAD binding site</scene>. | ||
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</StructureSection> | </StructureSection> | ||
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| - | ==3D structures of exoenzyme== | ||
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| - | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
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| - | *Exoenzyme C3 | ||
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| - | **[[1g24]] – CbExo residues 8-218 – ''Clostridium butulinum''<br /> | ||
| - | **[[1uzi]], [[1r45]], [[1r4b]], [[2c89]] – CbExo catalytic domain <br /> | ||
| - | **[[1gze]], [[2c8b]], [[2c8d]], [[2c8e]], [[2c8g]] – CbExo catalytic domain (mutant)<br /> | ||
| - | **[[3bw8]] - Exo catalytic domain - ''Clostridium limosum''<br /> | ||
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| - | *Exoenzyme C3 complexes | ||
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| - | **[[1gzf]] – CbExo catalytic domain + pyridinium derivative + ADP + NAD<br /> | ||
| - | **[[2bov]] – CbExo + GDP + Ral-A GTPase<br /> | ||
| - | **[[2a78]] – CbExo catalytic domain + GDP + Ral-A GTPase<br /> | ||
| - | **[[2a9k]] – CbExo catalytic domain+ GDP + Ral-A GTPase + NAD<br /> | ||
| - | **[[2c8a]] – CbExo catalytic domain + nicotinamide<br /> | ||
| - | **[[2c8c]] – CbExo catalytic domain (mutant) + ADP + NAD<br /> | ||
| - | **[[2c8f]], [[2c8h]] – CbExo catalytic domain (mutant) + NAD<br /> | ||
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| - | *Exoenzyme S | ||
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| - | **[[1r4t]] – PaExo S GAP domain – ''Pseudomonas aeruginosa'' - NMR<br /> | ||
| - | **[[1he1]] – PaExo S GAP domain + Ras-like protein + GDP <br /> | ||
| - | **[[1he9]] – PaExo S GAP domain + Ras-like protein <br /> | ||
| - | **[[6gn0]], [[6gn8]], [[6gnj]] – PaExo S residues 235-457 (mutant) + 14-3-3 protein β/α<br /> | ||
| - | **[[6gnk]] – PaExo S residues 235-457 (mutant) + 14-3-3 protein β/α + NAD derivative<br /> | ||
| - | **[[4zua]] – PaExo SA regulatory domain residues 2-178 <br /> | ||
| - | **[[3kxy]] – PaExo SC residues 1-133 + PaExo SE residues 16-81<br /> | ||
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| - | *Exoenzyme T | ||
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| - | **[[4jmf]] – PaExo residues 28-77 + chaperone<br /> | ||
| - | **[[6jnp]] – PaExo residues 23-79 + YOPE rgulator<br /> | ||
| - | **[[6gnn]] – PaExo residues 235-457 + 14-3-3 protein β/α<br /> | ||
| - | }} | ||
== References == | == References == | ||
Current revision
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References
- ↑ Han S, Arvai AS, Clancy SB, Tainer JA. Crystal structure and novel recognition motif of rho ADP-ribosylating C3 exoenzyme from Clostridium botulinum: structural insights for recognition specificity and catalysis. J Mol Biol. 2001 Jan 5;305(1):95-107. PMID:11114250 doi:10.1006/jmbi.2000.4292
- ↑ Menetrey J, Flatau G, Boquet P, Menez A, Stura EA. Structural basis for the NAD-hydrolysis mechanism and the ARTT-loop plasticity of C3 exoenzymes. Protein Sci. 2008 May;17(5):878-86. Epub 2008 Mar 27. PMID:18369192 doi:10.1110/ps.073398508
