This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
Exosome
From Proteopedia
(Difference between revisions)
| (4 intermediate revisions not shown.) | |||
| Line 2: | Line 2: | ||
<StructureSection load='2je6' size='340' side='right' caption='Exosome complex containing exonuclease 1 (green), exonuclease 2 (grey) and exosome complex RNA-binding protein 1 (wheat) complex with PEG400 (pdb code [[2je6]]).' scene=''> | <StructureSection load='2je6' size='340' side='right' caption='Exosome complex containing exonuclease 1 (green), exonuclease 2 (grey) and exosome complex RNA-binding protein 1 (wheat) complex with PEG400 (pdb code [[2je6]]).' scene=''> | ||
The '''Exosome complex''' (or just- Exosome) is a multi-protein complex capable of degrading various types of RNA molecules. The Exosome complex is found in eukaryotic cell, and also in archaea, while in bacteria it is found as a simpler complex (but it has the same function). <ref>http://en.wikipedia.org/wiki/Exosome_complex#Structure</ref> | The '''Exosome complex''' (or just- Exosome) is a multi-protein complex capable of degrading various types of RNA molecules. The Exosome complex is found in eukaryotic cell, and also in archaea, while in bacteria it is found as a simpler complex (but it has the same function). <ref>http://en.wikipedia.org/wiki/Exosome_complex#Structure</ref> | ||
| + | |||
| + | Some of the exosome components are:<br /> | ||
| + | *'''MTR3''' and '''SKI6''' are part of RNase PH domain-containing subunits proposed to form a central channel which threads RNA for degradation.<br /> | ||
| + | *'''MTR4''' interacts with Nop53 and together they have a role in the maturation of 5.8S rRNA<ref>PMID:28883156</ref>.<br /> | ||
| + | *'''Rrp6''' is a nuclear-specific subunit promoting cell survival during heat stress<ref>PMID:32521279</ref>.<br /> | ||
| + | *'''Rrp44''' is the catalytic subunit <ref>PMID:19060898</ref>.<br /> | ||
| + | *'''Csl4''' is required for mRNA decay.<br /> | ||
| + | *'''M-phase phosphoprotein 6''' is involved in the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA. | ||
| + | |||
__TOC__ | __TOC__ | ||
| Line 34: | Line 43: | ||
</StructureSection> | </StructureSection> | ||
| - | ==3D structures of exosome== | ||
| - | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
| - | |||
| - | *Exosome | ||
| - | |||
| - | **[[2cpr]] – hRRP6 HRDC domain – human - NMR<br /> | ||
| - | **[[3saf]], [[3sag]], [[3sah]] – hRRP6 catalytic domain (mutant)<br /> | ||
| - | **[[6ieg]] – hMTR4<br /> | ||
| - | **[[6ieh]] – hMTR4 + NRDE2<br /> | ||
| - | **[[2nn6]] – hCSL4 + RRP40 + RRP41 + RRP42 + RRP43 + RRP44 + RRP45 + RRP46 + RRP4 + MTR3 <br /> | ||
| - | **[[2je6]], [[2br2]] – SsRRP4 + RRP41 + RRP42 – ''Sulfolobus solfataricus'' <br /> | ||
| - | **[[2jeb]] - SsRRP4 + RRP41 + RRP42 + exonuclease 1 + RNA-binding protein 1 + Mn<br /> | ||
| - | **[[2ba0]] – AfRRP4 + RRP41 + RRP42 – Archaeoglobus fulgidus<br /> | ||
| - | **[[2ba1]] – AfCSL4 + RRP41 + RRP42 <br /> | ||
| - | **[[2hbj]], [[2hbk]] – yRRP6 (mutant) - yeast<br /> | ||
| - | **[[2ja9]] – yRRP6 <br /> | ||
| - | **[[2hbl]], [[2hbm]] – yRRP6 (mutant) + nucleotide<br /> | ||
| - | **[[4wfc]] – yRRP6 +RRP47 <br /> | ||
| - | **[[4wfd]] – yRRP6 +RRP47 + MTR4<br /> | ||
| - | **[[2wp8]] – yRRP45 + SKI6 + DIS3<br /> | ||
| - | **[[5okz]] – yCSL4 + RRP40 + RRP42 + RRP43 + RRP45 + RRP46 + RRP4 + SKI6 + MTR3 + M-phase phosphoprotein 6 <br /> | ||
| - | **[[5g06]] – yCSL4 + RRP40 + RRP42 + RRP43 + RRP45 + RRP46 + RRP4 + RRP6 + SKI6 + MTR3 + superkiller protein 7 + DIS3 <br /> | ||
| - | |||
| - | *Exosome complex with RNA | ||
| - | |||
| - | **[[6h25]] – hCSL4 + RRP40 + RRP41 + RRP42 + RRP43 + RRP44 + RRP45 + RRP46 + RRP4 + MTR3 + M-phase phosphoprotein 6 + RNA – Cryo EM<br /> | ||
| - | **[[6d6r]], [[6d6q]] – hCSL4 + RRP40 + RRP41 + RRP42 + RRP43 + RRP44 + RRP45 + RRP46 + RRP4 + MTR3 + MTR4 + component 10 + M-phase phosphoprotein 6 + DNA/RNA + RNA – Cryo EM<br /> | ||
| - | **[[2jea]] - SsRRP4 + RRP41 + RRP42 + exonuclease 1 + RNA-binding protein 1 + RNA<br /> | ||
| - | **[[3m7n]] – AfCSL4 + RRP41 + RRP42 + RNA<br /> | ||
| - | **[[2m85]] – AfCSL4 + RRP41 (mutant) + RRP42 (mutant) + RNA<br /> | ||
| - | **[[2vnu]], [[5c0y]] – yRRP6 + RNA<br /> | ||
| - | **[[4ifd]] – yCSL4 + RRP40 + RRP41 + RRP42 + RRP43 + RRP44 + RRP45 + RRP46 + RRP4 + MTR3 + RNA <br /> | ||
| - | **[[4oo1]] – yCSL4 + RRP40 + RRP42 + RRP43 + RRP45 + RRP46 + RRP4 + RRP6 + SKI6 + MTR3 + RNA <br /> | ||
| - | **[[5vzj]] – yCSL4 + RRP40 + RRP42 + RRP43 + RRP45 + RRP46 + RRP4 + RRP6 + SKI6 + MTR3 + M-phase phosphoprotein 6 + DIS3 + RNA <br /> | ||
| - | **[[5k36]], [[5c0x]], [[5c0w]] – yCSL4 + RRP40 + RRP42 + RRP43 + RRP45 + RRP46 + RRP4 + RRP6 + SKI6 + MTR3 + DIS3 + RNA <br /> | ||
| - | **[[5jea]] – yCSL4 + RRP40 + RRP42 + RRP43 + RRP45 + RRP46 + RRP4 + RRP6 + SKI6 + MTR3 + superkiller protein 7 + DIS3 + RNA <br /> | ||
| - | **[[5jea]] – yCSL4 + RRP40 + RRP42 + RRP43 + RRP45 + RRP46 + RRP4 + RRP6 + SKI6 + MTR3 + LRP1 + RNA <br /> | ||
| - | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category: Topic Page]] | [[Category: Topic Page]] | ||
Current revision
Exosome
| |||||||||||
References
- ↑ http://en.wikipedia.org/wiki/Exosome_complex#Structure
- ↑ Falk S, Tants JN, Basquin J, Thoms M, Hurt E, Sattler M, Conti E. Structural insights into the interaction of the nuclear exosome helicase Mtr4 with the pre-ribosomal protein Nop53. RNA. 2017 Sep 7. pii: rna.062901.117. doi: 10.1261/rna.062901.117. PMID:28883156 doi:http://dx.doi.org/10.1261/rna.062901.117
- ↑ Wang C, Liu Y, DeMario SM, Mandric I, Gonzalez-Figueroa C, Chanfreau GF. Rrp6 Moonlights in an RNA Exosome-Independent Manner to Promote Cell Survival and Gene Expression during Stress. Cell Rep. 2020 Jun 9;31(10):107754. PMID:32521279 doi:10.1016/j.celrep.2020.107754
- ↑ Schaeffer D, Tsanova B, Barbas A, Reis FP, Dastidar EG, Sanchez-Rotunno M, Arraiano CM, van Hoof A. The exosome contains domains with specific endoribonuclease, exoribonuclease and cytoplasmic mRNA decay activities. Nat Struct Mol Biol. 2009 Jan;16(1):56-62. doi: 10.1038/nsmb.1528. Epub 2008 Dec , 7. PMID:19060898 doi:http://dx.doi.org/10.1038/nsmb.1528
