Exportin

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<StructureSection load='3icq' size='400' side='right' caption='Structure of yeast exportin-5 (magenta) complex with GTPaseRan (cyan), Mg+2 (green) and RNA (PDB code [[3icq]]).' scene='59/592125/Cv/9'>
<StructureSection load='3icq' size='400' side='right' caption='Structure of yeast exportin-5 (magenta) complex with GTPaseRan (cyan), Mg+2 (green) and RNA (PDB code [[3icq]]).' scene='59/592125/Cv/9'>
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'''Exportins''' (XPO) or '''chromosomal maintenance protein''' mediate the nuclear export of cellular proteins with a leucine-rich export signal (NES) and RNA. XPO is regulated by GTPaseRan. RanGTP bind to XPO forming a ternary complex with the exported protein. The complex diffuses through the nuclear core complex to the cytoplasm and then dissociates. '''XPO-t''' exports tRNA from the nucleus<ref>PMID:17786152</ref>.
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'''Exportins''' (XPO) or '''chromosomal maintenance protein''' mediate the nuclear export of cellular proteins with a leucine-rich export signal (NES) and RNA. XPO is regulated by GTPaseRan. RanGTP bind to XPO forming a ternary complex with the exported protein. The complex diffuses through the nuclear core complex to the cytoplasm and then dissociates. <br />
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*'''XPO-1''' mediates nuclear transport of cellular proteins bearing a Leu-rich nuclear export signal and of RNA<ref>PMID:36727672</ref>.<br />
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*'''XPO-4''' mediates nuclear transport of eukaryotic translation initiation factor 5A<ref>PMID:10944119</ref>.<br />
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*'''XPO-5''' mediates nuclear transport of pre-miroRNAs and short hairpin RNAs<ref>PMID:14681208</ref>.<br />
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*'''XPO-t''' or '''karyopherin''' is a nuclear transport receptor involved in the export of tRNA to the cytoplasm<ref>PMID:22944664</ref>.<br />
==Structural highlights ==
==Structural highlights ==
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</StructureSection>
</StructureSection>
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==3D structures of exportin==
 
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
 
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{{#tree:id=OrganizedByTopic|openlevels=0|
 
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*XPO-1
 
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**[[4bsm]], [[4bsn]] – hXPO – human<br />
 
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**[[1w9c]] – hXPO C terminal 6 heat repeats <br />
 
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**[[3vyc]] – yXPO (mutant) - yeast<br />
 
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**[[4fgv]] – XPO – ''Chaetomium thermophilum''<br />
 
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*XPO-1 complexes
 
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**[[3gb8]] – hXPO + RNA U transporter 1 <br />
 
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**[[3gjx]] – hXPO + RNA U transporter 1 + RAN<br />
 
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**[[5dis]] – hXPO + RAN + RNA U transporter 1 + MBP<br />
 
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**[[3m1i]], [[4gpt]], [[4gmx]], [[4hat]], [[4hb2]], [[5jlj]] – yXPO (mutant) + RAN + RANBP1 <br />
 
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**[[3wyf]] – yXPO + RAN + RANBP1 <br />
 
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**[[3wyg]] – yXPO + RAN + PKI-α<br />
 
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**[[5zpu]], [[5ytb]], [[5yst]], [[5uro]] - yXPO (mutant) + RAN + RANBP1 + GTP<br />
 
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**[[5ysu]] - yXPO (mutant) + RAN + RANBP1 + GTP + plumbagin<br />
 
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**[[4hau]], [[4hav]], [[4haw]], [[4hax]], [[4hay]], [[4haz]], [[4hb0]], [[4hb3]], [[4hb4]] – yXPO (mutant) + RAN + RANBP1 + antibiotic<br />
 
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**[[5dh9]], [[5dha]], [[5dhf]], [[5di9]], [[5uwj]], [[5dif]] - yXPO (mutant) + RAN + RANBP1 + signal peptide<br />
 
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**[[5uwo]], [[5uwp]], [[5uwq]], [[5uwr]], [[5uws]], [[5uwt]], [[5uwu]], [[5uww]], [[5uwi]], [[5uwh]] - yXPO + RAN + RANBP1 + signal peptide<br />
 
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**[[6cit]], [[6a38]] - yXPO + RAN + RANBP1 + NS2 peptide<br />
 
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**[[6a3a]], [[6a3b]], [[6a3c]], [[6a3e]] - yXPO (mutant) + RAN + RANBP1 + NS2 peptide<br />
 
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**[[5xoj]] - yXPO (mutant) + GSP1P + PKI-a + NUP42 peptide + GTP<br />
 
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**[[3nby]], [[3nbz]], [[3nc0]] – mXPO + RNA U transporter 1 + RANBP1 - mouse<br />
 
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**[[3nc1]] – mXPO + RANBP1<br />
 
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**[[2l1l]] – hXPO residues 504-630 + PKI-α - NMR<br />
 
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*XPO-t
 
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**[[3ibv]] – fyXPO – fission yeast<br />
 
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**[[3icq]] – fyXPO + RAN homolog + RNA <br />
 
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*XPO-4
 
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**[[5dlq]] – mXPO (mutant) + RAN + EIF5A1<br />
 
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*XPO-5
 
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**[[5yu7]] – hXPO <br />
 
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**[[5yu6]] – hXPO + peptide + RAN <br />
 
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**[[3a6p]] – hXPO + peptide + pre-microRNA <br />
 
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}}
 
== References ==
== References ==

Current revision

Structure of yeast exportin-5 (magenta) complex with GTPaseRan (cyan), Mg+2 (green) and RNA (PDB code 3icq).

Drag the structure with the mouse to rotate

References

  1. Caillot M, Miloudi H, Taly A, Profitós-Pelejà N, Santos JC, Ribeiro ML, Maitre E, Saule S, Roué G, Jardin F, Sola B. Exportin 1-mediated nuclear/cytoplasmic trafficking controls drug sensitivity of classical Hodgkin's lymphoma. Mol Oncol. 2023 Dec;17(12):2546-2564. PMID:36727672 doi:10.1002/1878-0261.13386
  2. Lipowsky G, Bischoff FR, Schwarzmaier P, Kraft R, Kostka S, Hartmann E, Kutay U, Gorlich D. Exportin 4: a mediator of a novel nuclear export pathway in higher eukaryotes. EMBO J. 2000 Aug 15;19(16):4362-71. PMID:10944119 doi:http://dx.doi.org/10.1093/emboj/19.16.4362
  3. Yi R, Qin Y, Macara IG, Cullen BR. Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs. Genes Dev. 2003 Dec 15;17(24):3011-6. Epub 2003 Dec 17. PMID:14681208 doi:http://dx.doi.org/10.1101/gad.1158803
  4. Leisegang MS, Martin R, Ramírez AS, Bohnsack MT. Exportin t and Exportin 5: tRNA and miRNA biogenesis Biol Chem. 2012 Jul;393(7):599-604. PMID:22944664 doi:10.1515/hsz-2012-0146
  5. Cook AG, Fukuhara N, Jinek M, Conti E. Structures of the tRNA export factor in the nuclear and cytosolic states. Nature. 2009 Sep 3;461(7260):60-5. PMID:19680239 doi:10.1038/nature08394

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