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5a01

From Proteopedia

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O-GlcNAc transferase from Drososphila melanogaster

Structural highlights

5a01 is a 3 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.66Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q7KJA9_DROME

Publication Abstract from PubMed

Post-translational modification of intracellular proteins with O-linked N-acetylglucosamine (O-GlcNAc) catalysed by O-GlcNAc transferase (OGT) has been linked to regulation of diverse cellular functions. OGT possesses a C-terminal glycosyltransferase catalytic domain and N-terminal tetratricopeptide repeats that are implicated in protein-protein interactions. Drosophila OGT (DmOGT) is encoded by super sex combs (sxc), mutants of which are pupal lethal. However, it is not clear if this phenotype is caused by reduction of O-GlcNAcylation. Here we use a genetic approach to demonstrate that post-pupal Drosophila development can proceed with negligible OGT catalysis, while early embryonic development is OGT activity-dependent. Structural and enzymatic comparison between human OGT (hOGT) and DmOGT informed the rational design of DmOGT point mutants with a range of reduced catalytic activities. Strikingly, a severely hypomorphic OGT mutant complements sxc pupal lethality. However, the hypomorphic OGT mutant-rescued progeny do not produce F2 adults, because a set of Hox genes is de-repressed in F2 embryos, resulting in homeotic phenotypes. Thus, OGT catalytic activity is required up to late pupal stages, while further development proceeds with severely reduced OGT activity.

Dual functionality of O-GlcNAc transferase is required for Drosophila development.,Mariappa D, Zheng X, Schimpl M, Raimi O, Ferenbach AT, Muller HA, van Aalten DM Open Biol. 2015 Dec;5(12):150234. doi: 10.1098/rsob.150234. PMID:26674417^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mariappa D, Zheng X, Schimpl M, Raimi O, Ferenbach AT, Muller HA, van Aalten DM. Dual functionality of O-GlcNAc transferase is required for Drosophila development. Open Biol. 2015 Dec;5(12):150234. doi: 10.1098/rsob.150234. PMID:26674417 doi:http://dx.doi.org/10.1098/rsob.150234

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5a01"

@@ Line 3: / Line 3: @@
 <StructureSection load='5a01' size='340' side='right'caption='[[5a01]], [[Resolution|resolution]] 2.66&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5a01]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A01 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A01 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5a01]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A01 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A01 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=12V:(2S,3R,4R,5S,6R)-3-(acetylamino)-4,5-dihydroxy-6-(hydroxymethyl)tetrahydro-2H-thiopyran-2-yl+[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl+dihydrogen+diphosphate'>12V</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.66&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a01 OCA], [http://pdbe.org/5a01 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a01 RCSB], [http://www.ebi.ac.uk/pdbsum/5a01 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a01 ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=12V:(2S,3R,4R,5S,6R)-3-(acetylamino)-4,5-dihydroxy-6-(hydroxymethyl)tetrahydro-2H-thiopyran-2-yl+[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl+dihydrogen+diphosphate'>12V</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a01 OCA], [https://pdbe.org/5a01 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a01 RCSB], [https://www.ebi.ac.uk/pdbsum/5a01 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a01 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q7KJA9_DROME Q7KJA9_DROME]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 18: / Line 21: @@
 ==See Also==
-*[[Glycosyltransferase|Glycosyltransferase]]
+*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
-*[[O-GlcNAc transferase|O-GlcNAc transferase]]
+*[[O-GlcNAc transferase 3D structures|O-GlcNAc transferase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Drome]]
+[[Category: Drosophila melanogaster]]
 [[Category: Large Structures]]
-[[Category: Aalten, D M.F van]]
+[[Category: Ferenbach AT]]
-[[Category: Ferenbach, A T]]
+[[Category: Mariappa D]]
-[[Category: Mariappa, D]]
+[[Category: Mueller HJ]]
-[[Category: Mueller, H J]]
+[[Category: Rafie K]]
-[[Category: Rafie, K]]
+[[Category: Raimi O]]
-[[Category: Raimi, O]]
+[[Category: Schimpl M]]
-[[Category: Schimpl, M]]
+[[Category: Zheng X]]
-[[Category: Zheng, X]]
+[[Category: Van Aalten DMF]]
-[[Category: Embryonic development]]
-[[Category: Glycosyltransferase]]
-[[Category: Gt41]]
-[[Category: N-acetylglucosaminyltransferase]]
-[[Category: O-glcnac]]
-[[Category: Protein posttranslational modification]]
-[[Category: Tetratricopeptide repeat]]
-[[Category: Transferase]]

5a01

From Proteopedia

Current revision

O-GlcNAc transferase from Drososphila melanogaster

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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