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| | <StructureSection load='4zu5' size='340' side='right'caption='[[4zu5]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='4zu5' size='340' side='right'caption='[[4zu5]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4zu5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"clostridium_tartarivorum"_mercer_and_vaughn "clostridium tartarivorum" mercer and vaughn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZU5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZU5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4zu5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZU5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZU5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PXN:(2S)-1-[3-{[(2R)-2-HYDROXYPROPYL]OXY}-2,2-BIS({[(2R)-2-HYDROXYPROPYL]OXY}METHYL)PROPOXY]PROPAN-2-OL'>PXN</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4zu4|4zu4]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PXN:(2S)-1-[3-{[(2R)-2-HYDROXYPROPYL]OXY}-2,2-BIS({[(2R)-2-HYDROXYPROPYL]OXY}METHYL)PROPOXY]PROPAN-2-OL'>PXN</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">qdtA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1517 "Clostridium tartarivorum" Mercer and Vaughn])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zu5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zu5 OCA], [https://pdbe.org/4zu5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zu5 RCSB], [https://www.ebi.ac.uk/pdbsum/4zu5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zu5 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zu5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zu5 OCA], [http://pdbe.org/4zu5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zu5 RCSB], [http://www.ebi.ac.uk/pdbsum/4zu5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zu5 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q6TFC5_THETR Q6TFC5_THETR] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Clostridium tartarivorum mercer and vaughn]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Gilbert, M]] | + | [[Category: Thermoanaerobacterium thermosaccharolyticum]] |
| - | [[Category: Holden, H M]] | + | [[Category: Gilbert M]] |
| - | [[Category: Salinger, A J]] | + | [[Category: Holden HM]] |
| - | [[Category: Thoden, J B]] | + | [[Category: Salinger AJ]] |
| - | [[Category: Vinogradov, E]] | + | [[Category: Thoden JB]] |
| - | [[Category: 4-ketoisomerase]] | + | [[Category: Vinogradov E]] |
| - | [[Category: Cupin]]
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| - | [[Category: Isomerase]]
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| Structural highlights
Function
Q6TFC5_THETR
Publication Abstract from PubMed
3-Acetamido-3,6-dideoxy-d-galactose (Fuc3NAc) and 3-acetamido-3,6-dideoxy-d-glucose (Qui3NAc) are unusual sugars found on the lipopolysaccharides of Gram-negative bacteria and on the S-layers of Gram-positive bacteria. The 3,4-ketoisomerases, referred to as FdtA and QdtA, catalyze the third steps in the respective biosynthetic pathways for these sugars. Whereas both enzymes utilize the same substrate, the stereochemistries of their products are different. Specifically, the hydroxyl groups at the hexose C-4' positions assume the "galactose" and "glucose" configurations in the FdtA and QdtA products, respectively. In 2007 we reported the structure of the apoform of FdtA from Aneurinibacillus thermoaerophilus, which was followed in 2014 by the X-ray analysis of QdtA from Thermoanaerobacterium thermosaccharolyticum as a binary complex. Both of these enzymes belong to the cupin superfamily. Here we report a combined structural and enzymological study to explore the manner in which these enzymes control the stereochemistry of their products. Various site-directed mutant proteins of each enzyme were constructed, and their dTDP-sugar products were analyzed by NMR spectroscopy. In addition, the kinetic parameters for these protein variants were measured, and the structure of one, namely, the QdtA Y17R/R97H double mutant form, was determined to 2.3-A resolution. Finally, in an attempt to obtain a model of FdtA with a bound dTDP-linked sugar, the 3,4-ketoisomerase domain of a bifunctional enzyme from Shewanella denitrificans was cloned, purified, and crystallized in the presence of a dTDP-linked sugar analogue. Taken together, the results from this investigation demonstrate that it is possible to convert a "galacto" enzyme into a "gluco" enzyme and vice versa.
Bacterial Sugar 3,4-Ketoisomerases: Structural Insight into Product Stereochemistry.,Thoden JB, Vinogradov E, Gilbert M, Salinger AJ, Holden HM Biochemistry. 2015 Jul 13. PMID:26125548[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Thoden JB, Vinogradov E, Gilbert M, Salinger AJ, Holden HM. Bacterial Sugar 3,4-Ketoisomerases: Structural Insight into Product Stereochemistry. Biochemistry. 2015 Jul 13. PMID:26125548 doi:http://dx.doi.org/10.1021/acs.biochem.5b00541
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