5a4x

From Proteopedia

(Difference between revisions)

Current revision

The crystal structure of Arabidopsis thaliana CAR4 in complex with two calcium ions and Zn

Structural highlights

5a4x is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CAR4_ARATH Mediates the transient calcium-dependent interaction of PYR/PYL/RCAR abscisic acid (ABA) receptors with the plasma membrane and thus regulates ABA sensitivity (PubMed:26719420, PubMed:25465408). Stimulates the GTPase/ATPase activities of YchF1, and regulates its subcellular localization. Promotes tolerance towards salinity stress by limiting the accumulation of reactive oxygen species (ROS) (PubMed:23550829). Promotes resistance to bacterial pathogens (e.g. Xanthomonas oryzae pv. oryzae and P. syringae pv. tomato DC3000) (By similarity). Binds liposomes in the absence of exogenous Ca(2+), but this activity is enhanced in the presence of Ca(2+) and generates membrane curvature (PubMed:26719420).[UniProtKB:Q6YWF1]^[1] ^[2] ^[3]

Publication Abstract from PubMed

Regulation of ion transport in plants is essential for cell function. Abiotic stress unbalances cell ion homeostasis, and plants tend to readjust it, regulating membrane transporters and channels. The plant hormone abscisic acid (ABA) and the second messenger Ca(2+) are central in such processes, as they are involved in the regulation of protein kinases and phosphatases that control ion transport activity in response to environmental stimuli. The identification and characterization of the molecular mechanisms underlying the effect of ABA and Ca(2+) signaling pathways on membrane function are central and could provide opportunities for crop improvement. The C2-domain ABA-related (CAR) family of small proteins is involved in the Ca(2+)-dependent recruitment of the pyrabactin resistance 1/PYR1-like (PYR/PYL) ABA receptors to the membrane. However, to fully understand CAR function, it is necessary to define a molecular mechanism that integrates Ca(2+) sensing, membrane interaction, and the recognition of the PYR/PYL interacting partners. We present structural and biochemical data showing that CARs are peripheral membrane proteins that functionally cluster on the membrane and generate strong positive membrane curvature in a Ca(2+)-dependent manner. These features represent a mechanism for the generation, stabilization, and/or specific recognition of membrane discontinuities. Such structures may act as signaling platforms involved in the recruitment of PYR/PYL receptors and other signaling components involved in cell responses to stress.

Calcium-dependent oligomerization of CAR proteins at cell membrane modulates ABA signaling.,Diaz M, Sanchez-Barrena MJ, Gonzalez-Rubio JM, Rodriguez L, Fernandez D, Antoni R, Yunta C, Belda-Palazon B, Gonzalez-Guzman M, Peirats-Llobet M, Menendez M, Boskovic J, Marquez JA, Rodriguez PL, Albert A Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):E396-405. doi:, 10.1073/pnas.1512779113. Epub 2015 Dec 30. PMID:26719420^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cheung MY, Li MW, Yung YL, Wen CQ, Lam HM. The unconventional P-loop NTPase OsYchF1 and its regulator OsGAP1 play opposite roles in salinity stress tolerance. Plant Cell Environ. 2013 Nov;36(11):2008-20. doi: 10.1111/pce.12108. Epub 2013, Apr 25. PMID:23550829 doi:http://dx.doi.org/10.1111/pce.12108
↑ Rodriguez L, Gonzalez-Guzman M, Diaz M, Rodrigues A, Izquierdo-Garcia AC, Peirats-Llobet M, Fernandez MA, Antoni R, Fernandez D, Marquez JA, Mulet JM, Albert A, Rodriguez PL. C2-Domain Abscisic Acid-Related Proteins Mediate the Interaction of PYR/PYL/RCAR Abscisic Acid Receptors with the Plasma Membrane and Regulate Abscisic Acid Sensitivity in Arabidopsis. Plant Cell. 2014 Dec 2. pii: tpc.114.129973. PMID:25465408 doi:http://dx.doi.org/10.1105/tpc.114.129973
↑ Diaz M, Sanchez-Barrena MJ, Gonzalez-Rubio JM, Rodriguez L, Fernandez D, Antoni R, Yunta C, Belda-Palazon B, Gonzalez-Guzman M, Peirats-Llobet M, Menendez M, Boskovic J, Marquez JA, Rodriguez PL, Albert A. Calcium-dependent oligomerization of CAR proteins at cell membrane modulates ABA signaling. Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):E396-405. doi:, 10.1073/pnas.1512779113. Epub 2015 Dec 30. PMID:26719420 doi:http://dx.doi.org/10.1073/pnas.1512779113
↑ Diaz M, Sanchez-Barrena MJ, Gonzalez-Rubio JM, Rodriguez L, Fernandez D, Antoni R, Yunta C, Belda-Palazon B, Gonzalez-Guzman M, Peirats-Llobet M, Menendez M, Boskovic J, Marquez JA, Rodriguez PL, Albert A. Calcium-dependent oligomerization of CAR proteins at cell membrane modulates ABA signaling. Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):E396-405. doi:, 10.1073/pnas.1512779113. Epub 2015 Dec 30. PMID:26719420 doi:http://dx.doi.org/10.1073/pnas.1512779113

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5a4x"

Categories: Arabidopsis thaliana | Large Structures | Albert A | Diaz M

@@ Line 3: / Line 3: @@
 <StructureSection load='5a4x' size='340' side='right'caption='[[5a4x]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5a4x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A4X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A4X FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5a4x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A4X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A4X FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5a50|5a50]], [[5a51|5a51]], [[5a52|5a52]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a4x OCA], [http://pdbe.org/5a4x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a4x RCSB], [http://www.ebi.ac.uk/pdbsum/5a4x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a4x ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a4x OCA], [https://pdbe.org/5a4x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a4x RCSB], [https://www.ebi.ac.uk/pdbsum/5a4x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a4x ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/CAR4_ARATH CAR4_ARATH] Mediates the transient calcium-dependent interaction of PYR/PYL/RCAR abscisic acid (ABA) receptors with the plasma membrane and thus regulates ABA sensitivity (PubMed:26719420, PubMed:25465408). Stimulates the GTPase/ATPase activities of YchF1, and regulates its subcellular localization. Promotes tolerance towards salinity stress by limiting the accumulation of reactive oxygen species (ROS) (PubMed:23550829). Promotes resistance to bacterial pathogens (e.g. Xanthomonas oryzae pv. oryzae and P. syringae pv. tomato DC3000) (By similarity). Binds liposomes in the absence of exogenous Ca(2+), but this activity is enhanced in the presence of Ca(2+) and generates membrane curvature (PubMed:26719420).[UniProtKB:Q6YWF1]<ref>PMID:23550829</ref> <ref>PMID:25465408</ref> <ref>PMID:26719420</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Arath]]
+[[Category: Arabidopsis thaliana]]
 [[Category: Large Structures]]
-[[Category: Albert, A]]
+[[Category: Albert A]]
-[[Category: Diaz, M]]
+[[Category: Diaz M]]
-[[Category: C2 domain]]
-[[Category: Calcium binding protein]]
-[[Category: Lipid binding protein]]

5a4x

From Proteopedia

Current revision

The crystal structure of Arabidopsis thaliana CAR4 in complex with two calcium ions and Zn

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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