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| | ==SOLUTION STRUCTURE OF THE MAJOR CHERRY ALLERGEN PRU AV 1 MUTANT E45W== | | ==SOLUTION STRUCTURE OF THE MAJOR CHERRY ALLERGEN PRU AV 1 MUTANT E45W== |
| - | <StructureSection load='1h2o' size='340' side='right'caption='[[1h2o]], [[NMR_Ensembles_of_Models | 24 NMR models]]' scene=''> | + | <StructureSection load='1h2o' size='340' side='right'caption='[[1h2o]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1h2o]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H2O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H2O FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1h2o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Prunus_avium Prunus avium]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H2O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H2O FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1btv|1btv]], [[1b6f|1b6f]], [[1bv1|1bv1]], [[1e09|1e09]], [[1fsk|1fsk]], [[1qmr|1qmr]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h2o OCA], [http://pdbe.org/1h2o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1h2o RCSB], [http://www.ebi.ac.uk/pdbsum/1h2o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1h2o ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h2o OCA], [https://pdbe.org/1h2o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h2o RCSB], [https://www.ebi.ac.uk/pdbsum/1h2o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h2o ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/PRU1_PRUAV PRU1_PRUAV] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Boehm, M]] | + | [[Category: Prunus avium]] |
| - | [[Category: Lehmann, K]] | + | [[Category: Boehm M]] |
| - | [[Category: Nerkamp, J]] | + | [[Category: Lehmann K]] |
| - | [[Category: Neudecker, P]] | + | [[Category: Nerkamp J]] |
| - | [[Category: Roesch, P]] | + | [[Category: Neudecker P]] |
| - | [[Category: Scheurer, S]] | + | [[Category: Roesch P]] |
| - | [[Category: Schweimer, K]] | + | [[Category: Scheurer S]] |
| - | [[Category: Sticht, H]] | + | [[Category: Schweimer K]] |
| - | [[Category: Vieths, S]] | + | [[Category: Sticht H]] |
| - | [[Category: Allergen]]
| + | [[Category: Vieths S]] |
| - | [[Category: Heteronuclear nmr]]
| + | |
| - | [[Category: Major cherry allergen]]
| + | |
| - | [[Category: Pathogenesis-related protein]]
| + | |
| - | [[Category: Plant defense]]
| + | |
| Structural highlights
Function
PRU1_PRUAV
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Birch pollinosis is often accompanied by adverse reactions to food due to pollen-allergen specific IgE cross-reacting with homologous food allergens. The tertiary structure of Pru av 1, the major cherry (Prunus avium) allergen, for example, is nearly identical with Bet v 1, the major birch (Betula verrucosa) pollen allergen. In order to define cross-reactive IgE epitopes, we generated and analysed mutants of Pru av 1 and Api g 1.0101, the major celery (Apium graveolens) allergen, by immunoblotting, EAST (enzyme allergosorbent test), CD and NMR spectroscopy. The mutation of Glu45 to Trp45 in the P-loop region, a known IgE epitope of Bet v 1, significantly reduced IgE binding to Pru av 1 in a subgroup of cherry-allergic patients. The backbone conformation of Pru av 1 wild-type is conserved in the three-dimensional structure of Pru av 1 Trp45, demonstrating that the side chain of Glu45 is involved in a cross-reactive IgE epitope. Accordingly, for a subgroup of celery-allergic patients, IgE binding to the homologous celery allergen Api g 1.0101 was enhanced by the mutation of Lys44 to Glu. The almost complete loss of IgE reactivity to the Pru av 1 Pro112 mutant is due to disruption of its tertiary structure. Neither the mutation Ala112 nor deletion of the C-terminal residues 155-159 influenced IgE binding to Pru av 1. In conclusion, the structure of the P-loop partially explains the cross-reactivity pattern, and modulation of IgE-binding by site-directed mutagenesis is a promising approach to develop hypo-allergenic variants for patient-tailored specific immunotherapy.
Mutational epitope analysis of Pru av 1 and Api g 1, the major allergens of cherry (Prunus avium) and celery (Apium graveolens): correlating IgE reactivity with three-dimensional structure.,Neudecker P, Lehmann K, Nerkamp J, Haase T, Wangorsch A, Fotisch K, Hoffmann S, Rosch P, Vieths S, Scheurer S Biochem J. 2003 Nov 15;376(Pt 1):97-107. PMID:12943529[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Neudecker P, Lehmann K, Nerkamp J, Haase T, Wangorsch A, Fotisch K, Hoffmann S, Rosch P, Vieths S, Scheurer S. Mutational epitope analysis of Pru av 1 and Api g 1, the major allergens of cherry (Prunus avium) and celery (Apium graveolens): correlating IgE reactivity with three-dimensional structure. Biochem J. 2003 Nov 15;376(Pt 1):97-107. PMID:12943529 doi:http://dx.doi.org/10.1042/BJ20031057
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