Isopentenyl-diphosphate delta-isomerase
From Proteopedia
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'''Isopentenyl-diphosphate delta-isomerase''' (IPPI) catalyzes the conversion of the un-reactive isopentenyl diphosphate (IPP) to the more reactive dimethylallyl diphosphate (DMAPP). 3,4-epoxy-3-methyl butyl diphosphate (EIPP) is an irreversible inhibitor of IPPI. IPPI participates in isoprenoid biosynthesis<ref>PMID:3022798</ref>. | '''Isopentenyl-diphosphate delta-isomerase''' (IPPI) catalyzes the conversion of the un-reactive isopentenyl diphosphate (IPP) to the more reactive dimethylallyl diphosphate (DMAPP). 3,4-epoxy-3-methyl butyl diphosphate (EIPP) is an irreversible inhibitor of IPPI. IPPI participates in isoprenoid biosynthesis<ref>PMID:3022798</ref>. | ||
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| + | See also [[Mevalonate pathway]] | ||
== Structural highlights == | == Structural highlights == | ||
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*Isopentenyl-diphosphate delta-isomerase | *Isopentenyl-diphosphate delta-isomerase | ||
| + | **[[2icj]] – hIPPI + Mg - human<br /> | ||
| + | **[[2dho]] – hIPPI 1 (mutant) + Mn <br /> | ||
**[[1i9a]], [[2vnq]] – EcIPPI + Mn – ''Escherichia coli''<br /> | **[[1i9a]], [[2vnq]] – EcIPPI + Mn – ''Escherichia coli''<br /> | ||
**[[1hx3]] – EcIPPI (mutant) + Mn<br /> | **[[1hx3]] – EcIPPI (mutant) + Mn<br /> | ||
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**[[2g74]] – EcIPPI (mutant) + Mg + Mn<br /> | **[[2g74]] – EcIPPI (mutant) + Mg + Mn<br /> | ||
**[[1hzt]] – EcIPPI <br /> | **[[1hzt]] – EcIPPI <br /> | ||
| - | **[[2icj]] – hIPPI + Mg - human<br /> | ||
| - | **[[2dho]] – hIPPI 1 (mutant) + Mn <br /> | ||
**[[1p0k]] – BsIPPI – ''Bacillus subtilis''<br /> | **[[1p0k]] – BsIPPI – ''Bacillus subtilis''<br /> | ||
**[[1p0n]] – BsIPPI + FMN<br /> | **[[1p0n]] – BsIPPI + FMN<br /> | ||
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*Isopentenyl-diphosphate delta-isomerase complex | *Isopentenyl-diphosphate delta-isomerase complex | ||
| + | **[[2i6k]] – hIPPI (mutant) + Mn + Mg + aminoethanol-PP<br /> | ||
| + | **[[2ick]] – hIPPI + Mn + dimethylallyl-PP<br /> | ||
| + | **[[2pny]] – hIPPI 2 + Mn + PP<br /> | ||
**[[1nfs]], [[2vnp]] – EcIPPI + Mn + Mg + dimethylamino-ethyl-PP<br /> | **[[1nfs]], [[2vnp]] – EcIPPI + Mn + Mg + dimethylamino-ethyl-PP<br /> | ||
**[[1pvf]] – EcIPPI + Mn + Mg + PP<br /> | **[[1pvf]] – EcIPPI + Mn + Mg + PP<br /> | ||
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**[[3b06]], [[2zrx]], [[2zrz]] – SsIPPI + FMN + Mg + DMAPP<br /> | **[[3b06]], [[2zrx]], [[2zrz]] – SsIPPI + FMN + Mg + DMAPP<br /> | ||
**[[3b03]], [[3b04]] – SsIPPI + Mg + inhibitor<br /> | **[[3b03]], [[3b04]] – SsIPPI + Mg + inhibitor<br /> | ||
| - | **[[2i6k]] – hIPPI (mutant) + Mn + Mg + aminoethanol-PP<br /> | ||
| - | **[[2ick]] – hIPPI + Mn + dimethylallyl-PP<br /> | ||
| - | **[[2pny]] – hIPPI 2 + Mn + PP<br /> | ||
}} | }} | ||
Current revision
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3D structures of isopentenyl-diphosphate delta-isomerase
Updated on 16-January-2023
References
- ↑ Reardon JE, Abeles RH. Mechanism of action of isopentenyl pyrophosphate isomerase: evidence for a carbonium ion intermediate. Biochemistry. 1986 Sep 23;25(19):5609-16. PMID:3022798
- ↑ Wouters J, Oudjama Y, Barkley SJ, Tricot C, Stalon V, Droogmans L, Poulter CD. Catalytic mechanism of Escherichia coli isopentenyl diphosphate isomerase involves Cys-67, Glu-116, and Tyr-104 as suggested by crystal structures of complexes with transition state analogues and irreversible inhibitors. J Biol Chem. 2003 Apr 4;278(14):11903-8. Epub 2003 Jan 22. PMID:12540835 doi:http://dx.doi.org/10.1074/jbc.M212823200
